LS-C815617

antibody from LSBio

Targeting: HIPK1 KIAA0630, MGC26642, MGC33446, MGC33548, Myak, Nbak2

Western blot (Data presented on provider website)

Immunocytochemistry (Recommended by provider)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

LS-C815617

Provider

LSBio

Product name

HIPK1 Antibody (Preservative Free) LS-C815617

Antibody type

Polyclonal

Description

Protein A affinity chromatography

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

HIPK1 was immunoprecipitated using: Lane A: 0.5 mg NIH3T3 Whole Cell Lysate. Lane B: 0.5 mg A549 Whole Cell Lysate. 1 uL anti-HIPK1 rabbit polyclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-HIPK1 rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 133 kDa. Observed band size: 105 kDa.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

HIPK1 was immunoprecipitated using: Lane A: 0.5 mg NIH3T3 Whole Cell Lysate. Lane B: 0.5 mg A549 Whole Cell Lysate. 1 uL anti-HIPK1 rabbit polyclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-HIPK1 rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 133 kDa. Observed band size: 105 kDa.