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- Antibody Data
- Antigen structure
- References [11]
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- Western blot [1]
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- Product number
- MAB360-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human CCL20/MIP-3 alpha Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects human CCL20/MIP-3 alpha in ELISAs and Western blots. In ELISAs, no cross-reactivity with recombinant human (rh) CCL19, rhCXCL1, rhCCL16, rhCCL11, recombinant mouse CCL20/MIP-3 alpha , or recombinant rat CCL20/MIP-3 alpha is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P78556.1- Isotype
- IgG
- Antibody clone number
- 67310
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Circulating CCL20 as a New Biomarker of Abdominal Aortic Aneurysm.
Autophagy facilitates TLR4- and TLR3-triggered migration and invasion of lung cancer cells through the promotion of TRAF6 ubiquitination.
IL-22, but not IL-17, dominant environment in cutaneous T-cell lymphoma.
Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
Low expression of the IL-23/Th17 pathway in atopic dermatitis compared to psoriasis.
Expression of the Epstein-Barr virus-encoded Epstein-Barr virus nuclear antigen 1 in Hodgkin's lymphoma cells mediates Up-regulation of CCL20 and the migration of regulatory T cells.
Pre-eclampsia is associated with dendritic cell recruitment into the uterine decidua.
Trapping and apoptosis of novel subsets of memory T lymphocytes expressing CCR6 in the spleen of HIV-infected patients.
Substance P regulates macrophage inflammatory protein 3alpha/chemokine C-C ligand 20 (CCL20) with heme oxygenase-1 in human periodontal ligament cells.
Th2 cytokine response in Major Depressive Disorder patients before treatment.
Hypoxia modifies the transcriptome of primary human monocytes: modulation of novel immune-related genes and identification of CC-chemokine ligand 20 as a new hypoxia-inducible gene.
Soto B, Gallastegi-Mozos T, Rodríguez C, Martínez-González J, Escudero JR, Vila L, Camacho M
Scientific reports 2017 Dec 11;7(1):17331
Scientific reports 2017 Dec 11;7(1):17331
Autophagy facilitates TLR4- and TLR3-triggered migration and invasion of lung cancer cells through the promotion of TRAF6 ubiquitination.
Zhan Z, Xie X, Cao H, Zhou X, Zhang XD, Fan H, Liu Z
Autophagy 2014 Feb;10(2):257-68
Autophagy 2014 Feb;10(2):257-68
IL-22, but not IL-17, dominant environment in cutaneous T-cell lymphoma.
Miyagaki T, Sugaya M, Suga H, Kamata M, Ohmatsu H, Fujita H, Asano Y, Tada Y, Kadono T, Sato S
Clinical cancer research : an official journal of the American Association for Cancer Research 2011 Dec 15;17(24):7529-38
Clinical cancer research : an official journal of the American Association for Cancer Research 2011 Dec 15;17(24):7529-38
Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
Hu H, Kwun J, Aizenstein BD, Knechtle SJ
Transplantation 2009 Jun 27;87(12):1814-20
Transplantation 2009 Jun 27;87(12):1814-20
Low expression of the IL-23/Th17 pathway in atopic dermatitis compared to psoriasis.
Guttman-Yassky E, Lowes MA, Fuentes-Duculan J, Zaba LC, Cardinale I, Nograles KE, Khatcherian A, Novitskaya I, Carucci JA, Bergman R, Krueger JG
Journal of immunology (Baltimore, Md. : 1950) 2008 Nov 15;181(10):7420-7
Journal of immunology (Baltimore, Md. : 1950) 2008 Nov 15;181(10):7420-7
Expression of the Epstein-Barr virus-encoded Epstein-Barr virus nuclear antigen 1 in Hodgkin's lymphoma cells mediates Up-regulation of CCL20 and the migration of regulatory T cells.
Baumforth KR, Birgersdotter A, Reynolds GM, Wei W, Kapatai G, Flavell JR, Kalk E, Piper K, Lee S, Machado L, Hadley K, Sundblad A, Sjoberg J, Bjorkholm M, Porwit AA, Yap LF, Teo S, Grundy RG, Young LS, Ernberg I, Woodman CB, Murray PG
The American journal of pathology 2008 Jul;173(1):195-204
The American journal of pathology 2008 Jul;173(1):195-204
Pre-eclampsia is associated with dendritic cell recruitment into the uterine decidua.
Huang SJ, Chen CP, Schatz F, Rahman M, Abrahams VM, Lockwood CJ
The Journal of pathology 2008 Feb;214(3):328-36
The Journal of pathology 2008 Feb;214(3):328-36
Trapping and apoptosis of novel subsets of memory T lymphocytes expressing CCR6 in the spleen of HIV-infected patients.
Lécureuil C, Combadière B, Mazoyer E, Bonduelle O, Samri A, Autran B, Debré P, Combadière C
Blood 2007 May 1;109(9):3649-57
Blood 2007 May 1;109(9):3649-57
Substance P regulates macrophage inflammatory protein 3alpha/chemokine C-C ligand 20 (CCL20) with heme oxygenase-1 in human periodontal ligament cells.
Lee SK, Pi SH, Kim SH, Min KS, Lee HJ, Chang HS, Kang KH, Kim HR, Shin HI, Lee SK, Kim EC
Clinical and experimental immunology 2007 Dec;150(3):567-75
Clinical and experimental immunology 2007 Dec;150(3):567-75
Th2 cytokine response in Major Depressive Disorder patients before treatment.
Pavón L, Sandoval-López G, Eugenia Hernández M, Loría F, Estrada I, Pérez M, Moreno J, Avila U, Leff P, Antón B, Heinze G
Journal of neuroimmunology 2006 Mar;172(1-2):156-65
Journal of neuroimmunology 2006 Mar;172(1-2):156-65
Hypoxia modifies the transcriptome of primary human monocytes: modulation of novel immune-related genes and identification of CC-chemokine ligand 20 as a new hypoxia-inducible gene.
Bosco MC, Puppo M, Santangelo C, Anfosso L, Pfeffer U, Fardin P, Battaglia F, Varesio L
Journal of immunology (Baltimore, Md. : 1950) 2006 Aug 1;177(3):1941-55
Journal of immunology (Baltimore, Md. : 1950) 2006 Aug 1;177(3):1941-55
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Recombinant Human CCL20/MIP-3 alpha by Western Blot. Western blot shows 100 ng of Recombinant Human CCL20/MIP-3 alpha (Catalog # 360-MP), Recombinant Mouse CCL20/MIP-3 alpha (Catalog # 760-M3) and Recombinant Rat CCL20/MIP-3 alpha (Catalog # 540-RM). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human CCL20/MIP-3 alpha Monoclonal Antibody (Catalog # MAB360) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for CCL20/MIP-3 alpha at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Chemotaxis Induced by CCL20/MIP-3 alpha and Neutralization by Human CCL20/MIP-3 alpha Antibody. Recombinant Human CCL20/MIP-3 alpha (Catalog # 360-MP) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR6 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL20/MIP-3 alpha (10 ng/mL) is neutralized (green line) by increasing concentrations of Human CCL20/MIP-3 alpha Monoclonal Antibody (Catalog # MAB360). The ND50 is typically 1-5 µg/mL.
