PA5-47517

antibody from Invitrogen Antibodies

Targeting: CCL20 CKb4, exodus-1, LARC, MIP-3a, SCYA20, ST38

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-47517

Provider

Invitrogen Antibodies

Product name

MIP-3 alpha Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant mouse Teck is observed, and less than 1% cross-reactivity with recombinant human MIP-3 beta is observed.

Concentration

0.2 mg/mL

References

Submitted references

DEPDC1 drives hepatocellular carcinoma cell proliferation, invasion and angiogenesis by regulating the CCL20/CCR6 signaling pathway.

Guo W, Li H, Liu H, Ma X, Yang S, Wang Z
Oncology reports 2019 Sep;42(3):1075-1089

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of MIP-3 alpha in immersion fixed human peripheral blood mononuclear cells (PBMCs) stumulated with LPS. Samples were incubated in MIP-3 alpha polyclonal antibody (Product # PA5-47517) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow) and counterstained with DAPI (blue).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of CCL20/MIP-3a was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stumulated with LPS using human CCL20/MIP-3a Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47517) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the 557-conjugated Anti-Goat IgG Secondary Antibody (yello and counterstained with DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of MIP-3 alpha in immersion fixed paraffin-embedded sections of human skin. Samples were incubated in MIP-3 alpha polyclonal antibody (Product # PA5-47517) using a dilution of 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Neutralization of MIP-3 alpha in human CCR6. Samples were incubated in MIP-3 alpha polyclonal antibody (Product # PA5-47517). Recombinant Human CCL20/MIP-3α chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR6 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin. Chemotaxis elicited by Recombinant Human CCL20/MIP-3α (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL20/MIP-3α Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 0.2-1.0 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 4. CCL20 and CCR6 are significantly increased in HCC tissues and cell lines. (A) RT-qPCR analysis of DEPDC1, CCL20 and CCR6 mRNA in HCC tissue and matched adjacent normal liver tissue. (B) Analysis of RT-qPCR results with significant Pearson correlation analysis of DEPDC1 with CCL20 and DEPDC1 with CCR6 in HCC. (C and D) Immunohistochemistry assays were used to determine the protein expression of DEPDC1, CCL20 and CCR6. Scale bar, 25 um. (E) The correlation of DEPDC1 with CCL20 and CCR6 was determined via Pearson's correlation analysis. (F) Western blotting was performed to determine the protein expression of CCL20 and CCR6 in four human HCC cell lines (Li-7, Huh-7, SNU-387 and Hep3B) and one normal human hepatic cell line (L02). *P