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LearnPAB16925
antibody from Abnova Corporation
Targeting: CCL20
CKb4, exodus-1, LARC, MIP-3a, SCYA20, ST38
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- PAB16925 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB16925, RRID:AB_10677209
- Product name
- CCL20 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against full length recombinant CCL20.
- Storage
- Store at 4°C on dry atmosphere.After reconstitution with 0.1 mL of deionized water, store at -20°C or lower.Aliquot to avoid repeated freezing and thawing.
Submitted references CCL20/CCR6 chemokine/receptor expression in bone tissue from osteoarthritis and rheumatoid arthritis patients: different response of osteoblasts in the two groups.
C-C chemokine receptor 6-regulated entry of TH-17 cells into the CNS through the choroid plexus is required for the initiation of EAE.
Induction of CCL20 production by Kaposi sarcoma-associated herpesvirus: role of viral FLICE inhibitory protein K13-induced NF-kappaB activation.
Lisignoli G, Manferdini C, Codeluppi K, Piacentini A, Grassi F, Cattini L, Filardo G, Facchini A
Journal of cellular physiology 2009 Oct;221(1):154-60
Journal of cellular physiology 2009 Oct;221(1):154-60
C-C chemokine receptor 6-regulated entry of TH-17 cells into the CNS through the choroid plexus is required for the initiation of EAE.
Reboldi A, Coisne C, Baumjohann D, Benvenuto F, Bottinelli D, Lira S, Uccelli A, Lanzavecchia A, Engelhardt B, Sallusto F
Nature immunology 2009 May;10(5):514-23
Nature immunology 2009 May;10(5):514-23
Induction of CCL20 production by Kaposi sarcoma-associated herpesvirus: role of viral FLICE inhibitory protein K13-induced NF-kappaB activation.
Punj V, Matta H, Schamus S, Yang T, Chang Y, Chaudhary PM
Blood 2009 May 28;113(22):5660-8
Blood 2009 May 28;113(22):5660-8
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot using CCL20 polyclonal antibody (Cat # PAB16925) shows detection of a band ~26 KDa in size corresponding to recombinant human CCL20 (Lane 1). The identity of thelower molecular weight band is unknown. After transfer, the membrane was blocked overnightwith 3% BSA in TBS followed by reaction with primary antibody at a 1 : 1,000 dilution. Detection occurred using peroxidase conjugated anti-Rabbit IgG secondary antibody diluted 1 : 40,000 inblocking buffer for 30 min at RT followed by reaction with Femto Max™ chemiluminescent substrate. Image was captured using Versa Doc™ MP 4000 imaging system (Bio-Rad).
