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- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
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- Product number
- AF-556-NA - Provider product page
- Provider
- R&D Systems
- Product name
- Rat beta-NGF Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects rat beta-NGF in ELISAs and Western blots. In ELISAs, less than 0.1% cross-reactivity with recombinant human (rh) beta-NGF, rhBDNF, recombinant rat (rr) GDNF, rhCNTF, rrGDNF R alpha , rrCNTF, rhGDNF, rhNT-3 and rhNT-4 is observed.
- Reactivity
- Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P25427- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Keratinocyte expression of inflammatory mediators plays a crucial role in substance P-induced acute and chronic pain.
Bradykinin and nerve growth factor play pivotal roles in muscular mechanical hyperalgesia after exercise (delayed-onset muscle soreness).
Electrical stimulation of sympathetic neurons induces autocrine/paracrine effects of NGF mediated by TrkA.
Wei T, Guo TZ, Li WW, Hou S, Kingery WS, Clark JD
Journal of neuroinflammation 2012 Jul 23;9:181
Journal of neuroinflammation 2012 Jul 23;9:181
Bradykinin and nerve growth factor play pivotal roles in muscular mechanical hyperalgesia after exercise (delayed-onset muscle soreness).
Murase S, Terazawa E, Queme F, Ota H, Matsuda T, Hirate K, Kozaki Y, Katanosaka K, Taguchi T, Urai H, Mizumura K
The Journal of neuroscience : the official journal of the Society for Neuroscience 2010 Mar 10;30(10):3752-61
The Journal of neuroscience : the official journal of the Society for Neuroscience 2010 Mar 10;30(10):3752-61
Electrical stimulation of sympathetic neurons induces autocrine/paracrine effects of NGF mediated by TrkA.
Saygili E, Schauerte P, Küppers F, Heck L, Weis J, Weber C, Schwinger RH, Hoffmann R, Schröder JW, Marx N, Rana OR
Journal of molecular and cellular cardiology 2010 Jul;49(1):79-87
Journal of molecular and cellular cardiology 2010 Jul;49(1):79-87
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- beta-NGF in Rat Brain. beta-NGF was detected in perfusion fixed frozen sections of rat brain using Rat beta-NGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-556-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS009) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by beta-NGF and Neutralization by Rat beta-NGF Antibody. Recombinant Rat beta-NGF (Catalog # 556-NG) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat beta-NGF (3 ng/mL) is neutralized (green line) by increasing concentrations of Rat beta-NGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-556-NA). The ND50 is typically 0.02-0.1 µg/mL.
