MA5-32067

antibody from Invitrogen Antibodies

Targeting: NGF NGFB

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-32067

Provider

Invitrogen Antibodies

Product name

NGF Recombinant Rabbit Monoclonal Antibody (SI79-01)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Reactivity

Human, Mouse, Rat, Zebrafish

Host

Rabbit

Isotype

IgG

Antibody clone number

SI79-01

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

4-Aminopyridine Induces Nerve Growth Factor to Improve Skin Wound Healing and Tissue Regeneration.

Jagadeeshaprasad MG, Govindappa PK, Nelson AM, Noble MD, Elfar JC
Biomedicines 2022 Jul 8;10(7)

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Resveratrol Inhibition of the WNT/β-Catenin Pathway following Discogenic Low Back Pain.

Genovese T, Impellizzeri D, D'Amico R, Cordaro M, Peritore AF, Crupi R, Gugliandolo E, Cuzzocrea S, Fusco R, Siracusa R, Di Paola R
International journal of molecular sciences 2022 Apr 7;23(8)

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Synergic Neuroprotection Between Ligusticum Chuanxiong Hort and Borneol Against Ischemic Stroke by Neurogenesis via Modulating Reactive Astrogliosis and Maintaining the Blood-Brain Barrier.

Yu B, Yao Y, Zhang X, Ruan M, Zhang Z, Xu L, Liang T, Lu J
Frontiers in pharmacology 2021;12:666790

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Circulating microRNAs Profile in Patients With Transthyretin Variant Amyloidosis.

Vita GL, Aguennouz M, Polito F, Oteri R, Russo M, Gentile L, Barbagallo C, Ragusa M, Rodolico C, Di Giorgio RM, Toscano A, Vita G, Mazzeo A
Frontiers in molecular neuroscience 2020;13:102

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of NGF in Hela cell lysate using a NGF Monoclonal antibody (Product # MA5-32067) at a dilution of 1:1,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of NGF in HepG2 cells using a NGF Monoclonal antibody (Product # MA5-32067) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of NGF in NIH/3T3 cells using a NGF Monoclonal antibody (Product # MA5-32067) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of NGF in Hela cells using a NGF Monoclonal antibody (Product # MA5-32067) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of NGF of paraffin-embedded Mouse liver tissue using a NGF Monoclonal antibody (Product #MA5-32067). Counter stained with hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of NGF of paraffin-embedded Mouse brain tissue using a NGF Monoclonal antibody (Product #MA5-32067). Counter stained with hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of NGF of paraffin-embedded Mouse thymus tissue using a NGF Monoclonal antibody (Product #MA5-32067). Counter stained with hematoxylin.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Resveratrol administration reduced pain-related signaling. Western blot analysis of: trkA ( A ), NGF ( B ) expression in the disc tissue; Western blot analysis of: trkA ( C ), NGF ( D ) expression in the spinal cord tissue. * p < 0.05 vs. CFA, ## p < 0.01 vs. sham, ** p < 0.01 vs. CFA, ### p < 0.001 vs. sham, *** p < 0.001 vs. CFA. Error bar shown as SEM.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 6 4-AP enhanced expression of transcription factors, neurotrophic factors, and neuropeptides associated with reinnervation. ( a ) Representative image of triple co-immunostaining of wound skin for the transcription factor SOX10 (green), neuropeptide substance-P (SP--yellow), nerve growth factor (NGF--red), and nuclear stain DAPI (blue) and dashed line denotes epidermal/dermal border. Scale bars = 50 mum. ( b - d ) Quantification of SOX10, substance-P, and NGF expressing cells in healed wounds. Data represents 20 images from 5 different mouse wounds and are shown as mean +- SEM, n = 5 animals per group, statistical significance indicated by asterisks (O = saline mouse data points, = 4-AP treated mouse data points, * = p between 0.01 and 0.05, ** = p between 0.01 and 0.001, and *** = p between 0.001 and 0.0002 vs. saline). ( e ) Representative image of Western blot of SOX10, NGF, and GAPDH. ( f , g ) Normalized integrated densities for SOX10 and NGF immunoblot. Mean +- SEM, n = 3 animals per group, statistical significance indicated by asterisks (O = saline mouse data points, = 4-AP treated mouse data points, ** = p between 0.01 and 0.001, and *** = p between 0.001 and 0.0002 vs. saline).