PA5-55231

antibody from Invitrogen Antibodies

Targeting: VANGL1 STB2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-55231

Provider

Invitrogen Antibodies

Product name

VANGL1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: DTESTYSGYS YYSSHSKKSH RQGERTRERH KSPRNKDGRG SEKSVTIQPP TGEPLLGNDS TRTEEVQDDN WGETTTAITG TSEHSISQED IARISKDMED SVGLDCKRY

Concentration

0.2 mg/mL

References

Submitted references

Deletions in VANGL1 are a risk factor for antibody-mediated kidney disease.

Jiang SH, Mercan S, Papa I, Moldovan M, Walters GD, Koina M, Fadia M, Stanley M, Lea-Henry T, Cook A, Ellyard J, McMorran B, Sundaram M, Thomson R, Canete PF, Hoy W, Hutton H, Srivastava M, McKeon K, de la Rúa Figueroa I, Cervera R, Faria R, D'Alfonso S, Gatto M, Athanasopoulos V, Field M, Mathews J, Cho E, Andrews TD, Kitching AR, Cook MC, Riquelme MA, Bahlo M, Vinuesa CG
Cell reports. Medicine 2021 Dec 21;2(12):100475

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of VANGL1 in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using a VANGL1 Polyclonal Antibody (Product # PA5-55231). Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of VANGL1 in control (vector only transfected HEK293T lysate) and VANGL1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a VANGL1 Polyclonal Antibody (Product # PA5-55231).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of VANGL1 in human cell line U-251 MG shows positivity in centrosome & nucleus but excluded from the nucleoli. Samples were probed using a VANGL1 Polyclonal Antibody (Product # PA5-55231).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of VANGL1 in MCF7 cells using VANGL1 Polyclonal Antibody (Product # PA5-55231) shows localization to plasma membrane.