PAB20427

antibody from Abnova Corporation

Targeting: HJURP DKFZp762E1312, FAKTS, hFLEG1, URLC9

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB20427

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB20427, RRID:AB_10965448

Product name

HJURP polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant HJURP.

Antigen sequence

TPVVQMATLTYETPQGLRIWGGRLIKERNEGEIQD
SSMKPADRTDGSVQAAAWGPELPSHRTVLGADSKS
GEVDATSDQEESVAWALAPAVPQSPLKNELRRKYL
TQVDILLQGAEYFECAGNRAGRDVRV


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with HJURP polyclonal antibody (Cat # PAB20427) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 with HJURP polyclonal antibody (Cat # PAB20427) at 1-4 ug/mL dilution shows positivity in nucleus, nucleoli.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human prostate with HJURP polyclonal antibody (Cat # PAB20427) shows strong cytoplasmic positivity in glandular cells at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)