Explore
Validate
Learn
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [20]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP1-30042 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-30042, RRID:AB_1968578
- Product name
- Rabbit Polyclonal Periostin/OSF-2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specific for the ~93 kDa periostin doublet in mouse lung extract. Consistent with the fact that this antibody is made against an alternatively spliced region of periostin, this antibody recognizes only the largest two of the three forms of periostin recognized on Western blots by the Pan periostin antibody and also shows a distinctive staining pattern by immunohistochemistry. The antibody works well for immunohistochemistry on paraformaldehyde-fixed sections with a simple antigen-retrieval protocol (incubate slides for 20 minutes at 90 degrees C in 10 mM sodium citrate (pH 6.0)/ 0.1 % Tween-20).
- Reactivity
- Human, Mouse, Rat, Chicken/Avian, Rabbit
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Periostin Mediates Condylar Resorption via the NF-κB-ADAMTS5 Pathway.
Deficiency of emerin contributes differently to the pathogenesis of skeletal and cardiac muscles in LmnaH222P/H222P mutant mice.
Self-Assembly of an Organized Cementum-Periodontal Ligament-Like Complex Using Scaffold-Free Tissue Engineering.
Human antigen R as a therapeutic target in pathological cardiac hypertrophy.
WWP2 regulates pathological cardiac fibrosis by modulating SMAD2 signaling.
Cardiac Fibrosis in Proteotoxic Cardiac Disease is Dependent Upon Myofibroblast TGF -β Signaling.
Cardiac Dysfunction in the Sigma 1 Receptor Knockout Mouse Associated With Impaired Mitochondrial Dynamics and Bioenergetics.
Specialized fibroblast differentiated states underlie scar formation in the infarcted mouse heart.
Loss of periostin ameliorates adipose tissue inflammation and fibrosis in vivo.
TGFBI functions similar to periostin but is uniquely dispensable during cardiac injury.
Intravenous bisphosphonate therapy does not thicken cementum or change periodontal ligaments of cancer patients.
Anatomical differences of the protein profile in the rabbit sclera during growth.
Thrombospondin expression in myofibers stabilizes muscle membranes.
Genetic lineage tracing defines myofibroblast origin and function in the injured heart.
Proteomic analysis of dystrophin deficiency and associated changes in the aged mdx-4cv heart model of dystrophinopathy-related cardiomyopathy.
Simultaneous Pathoproteomic Evaluation of the Dystrophin-Glycoprotein Complex and Secondary Changes in the mdx-4cv Mouse Model of Duchenne Muscular Dystrophy.
Label-free mass spectrometric analysis of the mdx-4cv diaphragm identifies the matricellular protein periostin as a potential factor involved in dystrophinopathy-related fibrosis.
Oxazolone and ethanol induce colitis in non-obese diabetic-severe combined immunodeficiency interleukin-2Rγ(null) mice engrafted with human peripheral blood mononuclear cells.
Epithelial-to-mesenchymal transition in epicardium is independent of Snail1.
Deletion of periostin reduces muscular dystrophy and fibrosis in mice by modulating the transforming growth factor-β pathway.
Fan B, Liu X, Chen X, Xu W, Zhao H, Yang C, Zhang S
Inflammation 2020 Apr;43(2):455-465
Inflammation 2020 Apr;43(2):455-465
Deficiency of emerin contributes differently to the pathogenesis of skeletal and cardiac muscles in LmnaH222P/H222P mutant mice.
Wada E, Kato M, Yamashita K, Kokuba H, Liang WC, Bonne G, Hayashi YK
PloS one 2019;14(8):e0221512
PloS one 2019;14(8):e0221512
Self-Assembly of an Organized Cementum-Periodontal Ligament-Like Complex Using Scaffold-Free Tissue Engineering.
Basu A, Rothermund K, Ahmed MN, Syed-Picard FN
Frontiers in physiology 2019;10:422
Frontiers in physiology 2019;10:422
Human antigen R as a therapeutic target in pathological cardiac hypertrophy.
Green LC, Anthony SR, Slone S, Lanzillotta L, Nieman ML, Wu X, Robbins N, Jones SM, Roy S, Owens AP 3rd, Aube J, Xu L, Lorenz JN, Blaxall BC, Rubinstein J, Benoit JB, Tranter M
JCI insight 2019 Feb 21;4(4)
JCI insight 2019 Feb 21;4(4)
WWP2 regulates pathological cardiac fibrosis by modulating SMAD2 signaling.
Chen H, Moreno-Moral A, Pesce F, Devapragash N, Mancini M, Heng EL, Rotival M, Srivastava PK, Harmston N, Shkura K, Rackham OJL, Yu WP, Sun XM, Tee NGZ, Tan ELS, Barton PJR, Felkin LE, Lara-Pezzi E, Angelini G, Beltrami C, Pravenec M, Schafer S, Bottolo L, Hubner N, Emanueli C, Cook SA, Petretto E
Nature communications 2019 Aug 9;10(1):3616
Nature communications 2019 Aug 9;10(1):3616
Cardiac Fibrosis in Proteotoxic Cardiac Disease is Dependent Upon Myofibroblast TGF -β Signaling.
Bhandary B, Meng Q, James J, Osinska H, Gulick J, Valiente-Alandi I, Sargent MA, Bhuiyan MS, Blaxall BC, Molkentin JD, Robbins J
Journal of the American Heart Association 2018 Oct 16;7(20):e010013
Journal of the American Heart Association 2018 Oct 16;7(20):e010013
Cardiac Dysfunction in the Sigma 1 Receptor Knockout Mouse Associated With Impaired Mitochondrial Dynamics and Bioenergetics.
Abdullah CS, Alam S, Aishwarya R, Miriyala S, Panchatcharam M, Bhuiyan MAN, Peretik JM, Orr AW, James J, Osinska H, Robbins J, Lorenz JN, Bhuiyan MS
Journal of the American Heart Association 2018 Oct 16;7(20):e009775
Journal of the American Heart Association 2018 Oct 16;7(20):e009775
Specialized fibroblast differentiated states underlie scar formation in the infarcted mouse heart.
Fu X, Khalil H, Kanisicak O, Boyer JG, Vagnozzi RJ, Maliken BD, Sargent MA, Prasad V, Valiente-Alandi I, Blaxall BC, Molkentin JD
The Journal of clinical investigation 2018 May 1;128(5):2127-2143
The Journal of clinical investigation 2018 May 1;128(5):2127-2143
Loss of periostin ameliorates adipose tissue inflammation and fibrosis in vivo.
Nakazeki F, Nishiga M, Horie T, Nishi H, Nakashima Y, Baba O, Kuwabara Y, Nishino T, Nakao T, Ide Y, Koyama S, Kimura M, Tsuji S, Sowa N, Yoshida S, Conway SJ, Yanagita M, Kimura T, Ono K
Scientific reports 2018 Jun 4;8(1):8553
Scientific reports 2018 Jun 4;8(1):8553
TGFBI functions similar to periostin but is uniquely dispensable during cardiac injury.
Schwanekamp JA, Lorts A, Sargent MA, York AJ, Grimes KM, Fischesser DM, Gokey JJ, Whitsett JA, Conway SJ, Molkentin JD
PloS one 2017;12(7):e0181945
PloS one 2017;12(7):e0181945
Intravenous bisphosphonate therapy does not thicken cementum or change periodontal ligaments of cancer patients.
de Pauli Paglioni M, Gomes Silva W, Pereira J, Migliorati CA, Lopes MA, de Almeida OP, Goes MF, Ribeiro AC, Brandão TB, Santos-Silva AR
Oral surgery, oral medicine, oral pathology and oral radiology 2017 May;123(5):591-599
Oral surgery, oral medicine, oral pathology and oral radiology 2017 May;123(5):591-599
Anatomical differences of the protein profile in the rabbit sclera during growth.
Liu Y, Zhang P, Pan J, D'Souza MA, Dufresne CP, Semba RD, Qian J, Edward DP
Experimental eye research 2017 Jan;154:53-63
Experimental eye research 2017 Jan;154:53-63
Thrombospondin expression in myofibers stabilizes muscle membranes.
Vanhoutte D, Schips TG, Kwong JQ, Davis J, Tjondrokoesoemo A, Brody MJ, Sargent MA, Kanisicak O, Yi H, Gao QQ, Rabinowitz JE, Volk T, McNally EM, Molkentin JD
eLife 2016 Sep 26;5
eLife 2016 Sep 26;5
Genetic lineage tracing defines myofibroblast origin and function in the injured heart.
Kanisicak O, Khalil H, Ivey MJ, Karch J, Maliken BD, Correll RN, Brody MJ, J Lin SC, Aronow BJ, Tallquist MD, Molkentin JD
Nature communications 2016 Jul 22;7:12260
Nature communications 2016 Jul 22;7:12260
Proteomic analysis of dystrophin deficiency and associated changes in the aged mdx-4cv heart model of dystrophinopathy-related cardiomyopathy.
Murphy S, Dowling P, Zweyer M, Mundegar RR, Henry M, Meleady P, Swandulla D, Ohlendieck K
Journal of proteomics 2016 Aug 11;145:24-36
Journal of proteomics 2016 Aug 11;145:24-36
Simultaneous Pathoproteomic Evaluation of the Dystrophin-Glycoprotein Complex and Secondary Changes in the mdx-4cv Mouse Model of Duchenne Muscular Dystrophy.
Murphy S, Henry M, Meleady P, Zweyer M, Mundegar RR, Swandulla D, Ohlendieck K
Biology 2015 Jun 10;4(2):397-423
Biology 2015 Jun 10;4(2):397-423
Label-free mass spectrometric analysis of the mdx-4cv diaphragm identifies the matricellular protein periostin as a potential factor involved in dystrophinopathy-related fibrosis.
Holland A, Dowling P, Meleady P, Henry M, Zweyer M, Mundegar RR, Swandulla D, Ohlendieck K
Proteomics 2015 Jul;15(13):2318-31
Proteomics 2015 Jul;15(13):2318-31
Oxazolone and ethanol induce colitis in non-obese diabetic-severe combined immunodeficiency interleukin-2Rγ(null) mice engrafted with human peripheral blood mononuclear cells.
Nolte T, Zadeh-Khorasani M, Safarov O, Rueff F, Gülberg V, Herbach N, Wollenberg A, Mueller T, Siebeck M, Wolf E, Gropp R
Clinical and experimental immunology 2013 May;172(2):349-62
Clinical and experimental immunology 2013 May;172(2):349-62
Epithelial-to-mesenchymal transition in epicardium is independent of Snail1.
Casanova JC, Travisano S, de la Pompa JL
Genesis (New York, N.Y. : 2000) 2013 Jan;51(1):32-40
Genesis (New York, N.Y. : 2000) 2013 Jan;51(1):32-40
Deletion of periostin reduces muscular dystrophy and fibrosis in mice by modulating the transforming growth factor-β pathway.
Lorts A, Schwanekamp JA, Baudino TA, McNally EM, Molkentin JD
Proceedings of the National Academy of Sciences of the United States of America 2012 Jul 3;109(27):10978-83
Proceedings of the National Academy of Sciences of the United States of America 2012 Jul 3;109(27):10978-83
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Periostin/OSF-2 Antibody [NBP1-30042] - Rat lung lysate showing specific immunolabeling of the ~ 93 kDa periostin protein doublet.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Periostin/OSF-2 Antibody [NBP1-30042] - Analysis of periostin in rabbit periosteum. Image courtesy of product review submitted by Samer Zaky.
