LS-B326

antibody from LSBio

Targeting: ALPI IAP

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-B326

Provider

LSBio

Product name

IHC-plus™ ALPI / Alkaline Phosphatase Antibody LS-B326

Antibody type

Polyclonal

Description

Purified IgG

Reactivity

Human

Host

Rabbit

Storage

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Anti-ALPI / Alkaline Phosphatase antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Blocking/Neutralizing

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Anti-Alkaline Phosphatase Antibody - Western Blot. Both the antiserum and IgG fractions of anti-Alkaline Phosphatase (Human Intestine) are shown to detect under reducing conditions of SDS-PAGE the 60000 dalton enzyme in cellular extracts. Approximately 10 ug of total protein is loaded per lane. A 1:5000 dilution of the primary antibody is used followed by detection using HRP Goat-a-Rabbit IgG [H&L] (LS-C60884) diluted 1:4000 and color development using 4-CN substrate until sufficient color develops. Other detection systems will yield similar results.