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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- 200-401-142-0100 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#200-401-142-0100, RRID:AB_2612125
- Product name
- Anti-ALKALINE PHOSPHATASE (Human Intestine) (RABBIT) Antibody - 200-401-142-0100
- Antibody type
- Polyclonal
- Vial size
- 100 µl
Submitted references Rab11a is required for apical protein localisation in the intestine.
Sobajima T, Yoshimura S, Iwano T, Kunii M, Watanabe M, Atik N, Mushiake S, Morii E, Koyama Y, Miyoshi E, Harada A
Biology open 2014 Dec 19;4(1):86-94
Biology open 2014 Dec 19;4(1):86-94
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Supportive validation
- Submitted by
- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Both the antiserum and IgG fractions of anti-Alkaline Phosphatase (Human Intestine) are shown to detect under reducing conditions of SDS-PAGE the 60,000 dalton enzyme in cellular extracts. Approximately 10 ug of total protein is loaded per lane. A 1:5,000 dilution of the primary antibody is used followed by detection using HRP Goat-a-Rabbit IgG [H&L] (611-1302) diluted 1:4,000 and color development using 4-CN substrate until sufficient color develops. Other detection systems will yield similar results.
- Validation comment
- Western Blot
