AP07542PU-N

antibody from OriGene

Targeting: ALPI IAP

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP07542PU-N

Provider

OriGene

Product name

Alkaline Phosphatase (ALPI) rabbit polyclonal antibody, Purified

Antibody type

Polyclonal

Description

Alkaline Phosphatase (ALPI) rabbit polyclonal antibody, Purified

Host

Rabbit

Conjugate

Unconjugated

Epitope

ALPI

Antibody clone number

NULL

Vial size

50 µl

Concentration

10 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western Blot. Both the antiserum and IgG fractions of?anti-Alkaline Phosphatase (Human Intestine) are shown to detect under reducing conditions?of SDS-PAGE the 60000 dalton enzyme in cellular extracts. Approximately 10 ug of total?protein is loaded per lane. A 1/5000 dilution of the primary antibody is used followed by?detection using HRP Goat-a-Rabbit IgG [H&L] diluted 1/4000 and color?development using 4-CN substrate until sufficient color develops. Other detection systems will?yield similar results.

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Formalin-Fixed Paraffin-Embedded Human Small intestine stained with Alkaline Phosphatase Antibody at 5 ug/ml after heat-induced antigen retrieval.

Validation comment

IHC