Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
- Other assay [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-22210 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Intestinal Alkaline Phosphatase Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji.
- Concentration
- 0.3 mg/mL
Submitted references Human ALPI deficiency causes inflammatory bowel disease and highlights a key mechanism of gut homeostasis.
Mechanisms Involved in the Development of the Chronic Gastrointestinal Syndrome in Nonhuman Primates after Total-Body Irradiation with Bone Marrow Shielding.
Parlato M, Charbit-Henrion F, Pan J, Romano C, Duclaux-Loras R, Le Du MH, Warner N, Francalanci P, Bruneau J, Bras M, Zarhrate M, Bègue B, Guegan N, Rakotobe S, Kapel N, De Angelis P, Griffiths AM, Fiedler K, Crowley E, Ruemmele F, Muise AM, Cerf-Bensussan N
EMBO molecular medicine 2018 Apr;10(4)
EMBO molecular medicine 2018 Apr;10(4)
Mechanisms Involved in the Development of the Chronic Gastrointestinal Syndrome in Nonhuman Primates after Total-Body Irradiation with Bone Marrow Shielding.
Shea-Donohue T, Fasano A, Zhao A, Notari L, Yan S, Sun R, Bohl JA, Desai N, Tudor G, Morimoto M, Booth C, Bennett A, Farese AM, MacVittie TJ
Radiation research 2016 Jun;185(6):591-603
Radiation research 2016 Jun;185(6):591-603
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of alkaline phosphatase (intestinal) using 30 µg of HepG2 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with an Alkaline phosphatase (intestinal) polyclonal antibody (Product # PA5-22210) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Intestinal Alkaline Phosphatase Polyclonal Antibody (Product # PA5-22210). Untreated (–) and treated (+) SW1116 whole cell extract (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Intestinal Alkaline Phosphatase Polyclonal Antibody (Product # PA5-22210) diluted at 1:5,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Intestinal Alkaline Phosphatase Polyclonal Antibody detects Alkaline phosphatase (intestinal) protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. Alkaline phosphatase (intestinal) stained by Intestinal Alkaline Phosphatase Polyclonal Antibody (Product # PA5-22210) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded SG xenograft, using alkaline phosphatase (intestinal) (Product # PA5-22210) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 Reduced ALPI expression in patients' small intestinal biopsies A, B Immunofluorescence microscopy of duodenum (A) and ileum (B) sections from Patient 1 (A) and Patient 2 (B) compared to normal or IBD controls. Sections were stained with DAPI (4',6-diamidino-2-phenylindole) for DNA (blue) and antibodies against ALPI alone (second column, red) or ALPI and E-cadherin (green) (first column, merge staining). Magnification 20x (scale bar = 100 mum). C Effect of AP inhibitors (L-phe, L-arg) on AP activity in stools of non-inflamed controls ( n = 20). Data are expressed as mean +- SD. **** P < 0.0001, nonparametric, unpaired two-tailed Mann-Whitney test. D ALPI activity in stools of non-inflamed ( n = 20) or IBD controls ( n = 14) and of P1. AP values are expressed as units of ALPI/g stool and as medians with interquartiles; * P = 0.0122, nonparametric, unpaired two-tailed Mann-Whitney test.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure EV4 TNAP expression in patients' small intestinal biopsies A, B Immunofluorescence microscopy of duodenum (A) and ileum (B) sections from P1 (A) and P2 (B) compared with normal or IBD controls. Sections were counterstained with blue RedDot2 dye for DNA and antibodies against ALPI alone (red), TNAP alone (green) or TNAP and ALPI (first column, merge staining). Magnification 20x (Scale bars: 100 mum).