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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB1899 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB1899, RRID:AB_1574638
- Product name
- EIF4E2 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of EIF4E2.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Characterizing the interaction of the mammalian eIF4E-related protein 4EHP with 4E-BP1.
Cloning and characterization of 4EHP, a novel mammalian eIF4E-related cap-binding protein.
Identification of genes expressed in human CD34(+) hematopoietic stem/progenitor cells by expressed sequence tags and efficient full-length cDNA cloning.
Tee AR, Tee JA, Blenis J
FEBS letters 2004 Apr 23;564(1-2):58-62
FEBS letters 2004 Apr 23;564(1-2):58-62
Cloning and characterization of 4EHP, a novel mammalian eIF4E-related cap-binding protein.
Rom E, Kim HC, Gingras AC, Marcotrigiano J, Favre D, Olsen H, Burley SK, Sonenberg N
The Journal of biological chemistry 1998 May 22;273(21):13104-9
The Journal of biological chemistry 1998 May 22;273(21):13104-9
Identification of genes expressed in human CD34(+) hematopoietic stem/progenitor cells by expressed sequence tags and efficient full-length cDNA cloning.
Mao M, Fu G, Wu JS, Zhang QH, Zhou J, Kan LX, Huang QH, He KL, Gu BW, Han ZG, Shen Y, Gu J, Yu YP, Xu SH, Wang YX, Chen SJ, Chen Z
Proceedings of the National Academy of Sciences of the United States of America 1998 Jul 7;95(14):8175-80
Proceedings of the National Academy of Sciences of the United States of America 1998 Jul 7;95(14):8175-80
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of EIF4E2 polyclonal antibody (Cat # PAB1899) (arrow). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EIF4E2 gene (Lane 2) (Origene Technologies).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human breast cancer tissue reacted with EIF4E2 polyclonal antibody (Cat # PAB1899) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
