12227-1-AP

antibody from Invitrogen Antibodies

Targeting: EIF4E2 4EHP, EIF4EL3, IF4e

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

12227-1-AP

Provider

Invitrogen Antibodies

Product name

EIF4E2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MNNKFDALK DDDSGDHDQN EENSTQKDGE KEKTERDKNQ SSSKRKAVVP GPAEHPLQYN YTFWYSRRTP GRPTSSQSYE QNIKQIGTFA SVEQFWRFYS HMVRPGDLTG HSDFHLFKEG IKPMWEDDAN KNGGKWIIRL RKGLASRCWE NLILAMLGEQ FMVGEEICGA VVSVRFQEDI ISIWNKTASD QATTARIRDT LRRVLNLPPN TIMEYKTHTD SIKMPGRLGP QRLLFQNLWK PRLNVP (1-245 aa encoded by B C021690)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.22 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

MCF7 cells were subjected to SDS PAGE followed by western blot with 12227-1-AP (EIF4E2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 12227-1-AP (EIF4E2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12227-1-AP (EIF4E2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

MCF7 cells were subjected to SDS PAGE followed by western blot with 12227-1-AP (EIF4E2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 12227-1-AP (EIF4E2 Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using 12227-1-AP (EIF4E2 Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using 12227-1-AP (EIF4E2 Antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 12227-1-AP ( EIF4E2 Antibody) at dilution of 1:50 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-EIF4E2 (IP:12227-1-AP, 4ug; Detection:12227-1-AP 1:500) with MCF-7 cells lysate 1280ug.