HPA019253

antibody from Atlas Antibodies

Targeting: EIF4E2 4EHP, EIF4EL3, IF4e

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA019253

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA019253, RRID:AB_1848092

Product name

Anti-EIF4E2

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

TERDKNQSSSKRKAVVPGPAEHPLQYNYTFWYSRR
TPGRPTSSQSYEQNIKQIGTFASVEQFWRFYSHMV
RPGDLTGHSDFHLFKEGIKPMWEDDANKNGGKWII
RL


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Recombinant expression validation

Main image

Experimental details

Western blot analysis in control (vector only transfected HEK293T lysate) and EIF4E2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY401518).

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis in human cell line RH-30.

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & mitochondria.

Sample type

HUMAN