PAB22069

antibody from Abnova Corporation

Targeting: CHD1L ALC1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB22069

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB22069, RRID:AB_10962779

Product name

CHD1L polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant CHD1L.

Antigen sequence

AWWESNNYQSFCLPSEESEPEDLENGEESSAELDY
QDPDATSLKYVSGDVTHPQAGAEDALIVHCVDDSG
HWGRGGLFTALEKRSAEPR


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with CHD1L polyclonal antibody (Cat # PAB22069) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 with CHD1L polyclonal antibody (Cat # PAB22069) at 1-4 ug/mL dilution shows positivity in nucleus but not nucleoli.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human testis with CHD1L polyclonal antibody (Cat # PAB22069) shows distinct nuclear positivity in germ cells of testis at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)