Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [3]
- Flow cytometry [1]
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Validation data
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- Product number
- MA5-34732 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CHD1L Recombinant Rabbit Monoclonal Antibody (JG35-44)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: HepG2, human tonsil tissue, human colon cancer tissue, mouse testis tissue, HepG2.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- JG35-44
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles, store in dark
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CHD1L in HepG2 cell. Samples were incubated with CHD1L monoclonal antibody (Product # MA5-34732), at a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CHD1L in paraffin-embedded mouse testis tissue. Samples were heat mediated antigen retrieval with sodium citrate buffer (pH6, 20 min), incubated with CHD1L monoclonal antibody (Product # MA5-34732), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CHD1L in paraffin-embedded human tonsil tissue. Samples were heat mediated antigen retrieval with sodium citrate buffer (pH6, 20 min), incubated with CHD1L monoclonal antibody (Product # MA5-34732), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CHD1L in paraffin-embedded human colon cancer tissue. Samples were heat mediated antigen retrieval with sodium citrate buffer (pH6, 20 min), incubated with CHD1L monoclonal antibody (Product # MA5-34732), and followed by hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of CHD1L in HepG2 cells (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Samples were incubated with CHD1L monoclonal antibody (Product # MA5-34732) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.