UM500062CF

antibody from Invitrogen Antibodies

Targeting: FCGR1A CD64, CD64A

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

UM500062CF

Provider

Invitrogen Antibodies

Product name

FCGR1A Monoclonal Antibody (UMAB74), UltraMAB™

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

UMAB74

Vial size

100 µg

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FCGR1A(RC207487, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-FCGR1A. (1:2000)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot of human tissue lysates (15 µg) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen). Dilution: 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of extracts (35 µg) from 1 cell line and 1 tissue lysates by using anti-FCGR1A monoclonal antibody. (1:500)

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded human breast tissue using anti-FCGR1A mouse monoclonal antibody. (UM500062; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)(1:100)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded human pancreas tissue using anti-FCGR1A mouse monoclonal antibody. (UM500062; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded human prostate tissue using anti-FCGR1A mouse monoclonal antibody. (UM500062; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-FCGR1A mouse monoclonal antibody. (UM500062; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded human Kidney tissue using anti-FCGR1A mouse monoclonal antibody. (UM500062; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)(1:100)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-FCGR1A mouse monoclonal antibody. (UM500062; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded human lung tissue using anti-FCGR1A mouse monoclonal antibody. (UM500062; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of living 293T cells transfected with FCGR1A overexpression plasmid (RC207487), Red)/empty vector (PS100001, Blue) using anti-FCGR1A antibody (UM500062). cells incubated with a non-specific antibody (Green) were used as isotype control. (1:100)

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-FCGR1A mouse monoclonal antibody (UM500062). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.