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LearnPA5-118065
antibody from Invitrogen Antibodies
Targeting: MLPH
exophilin-3, l(1)-3Rk, l1Rk3, ln, Slac-2a
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
- Other assay [2]
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- Product number
- PA5-118065 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MLPH Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using MLPH Polyclonal Antibody (Product # PA5-118065) at 1:500 dilution. Lane A: U-251 MG Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 66 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot of MLPH in Lane A: U-251 MG Whole Cell Lysate. Samples (30 µg per lane) were incubated with polyclonal antibody (Product # PA5-118065) with a dilution of 1:500 , followed by Goat Anti-Rabbit IgG (H+L)/HRP using a dilution of 1:10,000. Assay was performed under reducing conditions. Predicted band size: 66 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) of MLPH in human stomach. Samples were incubated with polyclonal antibody (Product # PA5-118065) with a dilution of 1:100 .
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) of MLPH in human gallbladder. Samples were incubated with polyclonal antibody (Product # PA5-118065) with a dilution of 1:100 .
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MLPH Immunoprecipitation using: Lane A: 0.5 mg U-251 MG Whole Cell Lysate 4 µL with MLPH Polyclonal Antibody (Product # PA5-118065) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: MLPH Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) /HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 66 kDa. Observed band size: 90 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of MLPH in Lane A: 0.5 mg U-251 MG Whole Cell Lysate. Samples were treated with 60 μg of immunomagnetic Protein A/G beads, incubated with polyclonal antibody (Product # PA5-118065) with a dilution of 1:100 , followed by Goat Anti-Rabbit IgG (H+L)/HRP using a dilution of 1:10,000. Assay was performed under reducing conditions. Predicted band size: 66 kDa , Observed band size : 90 kDa .
