M06414

antibody from Boster Biological Technology

Targeting: SF3B4 Hsh49, SAP49, SF3b49

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

M06414

Provider

Boster Biological Technology

Product name

Anti-Splicing Factor SF3B4 Monoclonal Antibody

Antibody type

Monoclonal

Description

Anti-Splicing Factor SF3B4 Monoclonal Antibody tested in WB, IF, ICC, IHC, reactive to Human, Rat, Mouse

Reactivity

Human, Mouse, Rat

Host

Mouse

Vial size

100ul

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

Store at 4℃ for short term, for longer term at -20℃

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of different cell lysates, cytosol or nuclear enriched fractions, using mouse mAb to splicing factor SF3B4, M06414, dilution 1:1,000 in green: [1] protein standard (red), [2] NIH-3T3 cytosolic fraction [3] NIH-3T3 nuclear fraction [4] HeLa cytosolic and [5] HeLa nuclear fractions. Strong single band at 49kDa represents the SF3B4 protein, which is expressed exclusively in the nuclei. The same blot was simultaneously probed with rabbit pAb to GAPDH, dilution 1:20,000, in red. The 37kDa band corresponds to the GAPDH protein, detected mainly in the cytosolic fractions of these cells.

Additional image