HPA027241

antibody from Atlas Antibodies

Targeting: ECM1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA027241

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA027241, RRID:AB_1847976

Product name

Anti-ECM1

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

EDTLDKYCDREYAVKTHHHLCCRHPPSPTRDECFA
RRAPYPNYDRDILTIDIGRVTPNLMGHLCGNQRVL
TKHKHIPGLIHNMTARCCDLPFPEQACCAEEEKLT
FINDLCGPRRNIWRDPALCCYLSPGDEQVNCFNIN
YLRNVALVS


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Western blot analysis in human cell lines SK-MEL-30 and MCF-7 using Anti-ECM1 antibody. Corresponding ECM1 RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.

Orthogonal method

Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.

Show more

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human epididymis and pancreas tissues using Anti-ECM1 antibody. Corresponding ECM1 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Show more

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human vagina shows strong cytoplasmic positivity in subset of squamous epithelial cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human epididymis shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human pancreas shows low expression as expected.

Sample type

HUMAN