MAB11329

antibody from Abnova Corporation

Targeting: ECM1

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB11329

Provider

Abnova Corporation

Product name

ECM1 monoclonal antibody, clone SC05

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against ECM1.

Isotype

IgG

Antibody clone number

SC05

Storage

Store at 4°C.Aliquot to avoid repeated freezing and thawing.

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemistry (Formalin-fixed paraffin-embedded sections) analysis of human small intestine with ECM1 monoclonal antibody, clone SC05 (Cat # MAB11329) at 1:500 using peroxidase-conjugate and DAB chromogen (Note: cytoplasmic and membrane staining). Immunohistochemical staining of formalin/paraffin tissues is enhanced by unmasking of the target epitope with trypsin at 1 mg/ml for 5 min at 37°C. Note: enzyme digestion may be better than citrate buffer based epitope unmasking according to Brown, 1993. However, we have found antigen retrieval with Heat Induced Epitope Retrieval (HIER) in Citrate buffer (pH6.0) also works well. In this case, de-paraffinized tissue sections should be boiled in this buffer for 5 minutes followed by cooling at room temp for 20 minutes.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)