AF3937

antibody from R&D Systems

Targeting: ECM1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF3937

Provider

R&D Systems

Product name

Human ECM1 Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects human ECM-1 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant mouse ECM-1 is observed.

Reactivity

Human

Host

Sheep

Conjugate

Unconjugated

Antigen sequence

AAH23505


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human ECM-1 by Western Blot. Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line and SK-Mel-28 human malignant melanoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human ECM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3937) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ECM-1 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human ECM-1 by Simple Western^TM. Simple Western lane view shows lysates of COLO 205 human colorectal adenocarcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for ECM-1 at approximately 90 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human ECM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3937) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

ECM-1 in Human Colon Cancer Tissue. ECM-1 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using 15 µg/mL Sheep Anti-Human ECM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3937) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of epithelial cells. Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.