ABIN2616342

antibody from antibodies-online

Targeting: ANKMY1 FLJ20499, ZMYND13

Western blot (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

ABIN2616342

Provider

antibodies-online

Product name

anti-Ankyrin Repeat and MYND Domain Containing 1 (ANKMY1) (AA 1-720) antibody

Antibody type

Polyclonal

Description

Affinity purified

Reactivity

Human

Host

Mouse

Epitope

AA 1-720

Isotype

IgG

Vial size

50 μg

Storage

May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.

Handling

Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Histone H3 dimethyl Arg8 asymmetric pAb tested by Western blot. Nuclear extract from U2OS cells (20 μg) or HEK293 cells (20 μg) was probed with Histone H3 dimethyl Arg8 asymmetric pAb (1:500 dilution).

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Histone H3 dimethyl Arg8 asymmetric pAb tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H3 dimethyl Arg8 asymmetric pAb for dimethyl-arginine 8 of histone H3. Peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with Histone H3 dimethyl Arg8 asymmetric pAb at 1:30,000. The amount of peptide (picomoles) spotted is indicated next to each row. Top Panel: Lane 1: unmod Arg2. Lane 2: monomethyl-Arg2 H3. Lane 3: dimethyl-Arg2 H3 (sym). Lane 4: dimethyl-Arg2 H3 (asym). Lane 5: unmod Arg8. Lane 6: monomethyl-Arg8 H3. Lane 7: dimethyl-Arg8 H3 (sym). Lane 8: dimethyl-Arg8 H3 (asym). Bottom Panel: Lane 1: unmod Arg17 H3. Lane 2: monomethyl-Arg17. Lane 3: dimethyl-Arg17 H3 (sym). Lane 4: dimethyl-Arg17 H3 (asym). Lane 5: unmod Arg26. Lane 6: monomethyl-Arg26 H3. Lane 7: dimethyl-Arg26 H3 (sym). Lane 8: dimethyl-Arg26 H3 (asymmetric). No detection of peptides (unmodified, mono-, di-, or tri-methylated) corresponding to Lys4, Lys9, Lys18, Lys23 and Lys27 of histone H3 was observed.

Chromatin Immunoprecipitation

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Histone H3R8me2a antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 ug of chromatin from a mouse osteoblast cell line and 7 μl of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 20 million sequence tags were mapped to identify Histone H3R8me2a binding sites. The image shows binding across a region of chromosome 5. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here.