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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [3]
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- Product number
- ARG54853 - Provider product page
- Provider
- Arigo
- Product name
- anti-Gremlin antibody
- Antibody type
- Polyclonal
- Antigen
- KLH-conjugated synthetic peptide around aa. 147-175 (C-terminus) of Human GREMLIN.
- Description
- Purification with Protein G.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µl
- Storage
- For continuous use, store undiluted antibody at 2-8°C for up to 6 months. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 35 µg of CEM cell line lysates stained with ARG54853 anti-Gremlin antibody.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: mouse liver cell lysate stained with ARG54853 anti-Gremlin antibody.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: formalin-PBS and Bouin embedded paraffin human renal tissue pretreated with heat induced epitope retrieval Citrate buffer pH 6.0 in microwave, stained with ARG54853 anti-Gremlin antibody at 1:50 dilution. Detection method was ABC system or Vectastaint Elite VECTOR.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: formalin-fixed and paraffin-embedded human skeletal muscle tissue stained with ARG54853 anti-Gremlin antibody.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: ISH and IMH demonstrating gremlin mRNA and gremlin protein in crescentic PECs and tubular epithelial cells of patients with pauci-immune crescentic GN. In comparison with normal renal tissue in which there is no expression of gremlin mRNA (C), proliferating PECs of glomerular crescents show a strong expression of gremlin mRNA by ISH (A); and gremlin protein expression by IMH (B); immune competent infiltrating interstitial cells are also strongly positive for gremlin staining (E); and CTGF was also expressed in these glomerular crescentic cells (F).
