14396-1-AP

antibody from Invitrogen Antibodies

Targeting: PASK KIAA0135, PASKIN, STK37

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

14396-1-AP

Provider

Invitrogen Antibodies

Product name

PASK Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: YSTMSPLGS GAFGFVWTAV DKEKNKEVVV KFIKKEKVLE DCWIEDPKLG KVTLEIAILS RVEHANIIKV LDIFENQGFF QLVMEKHGSG LDLFAFIDRH PRLDEPLASY IFRQLVSAVG YLRLKDIIHR DIKDENIVIA EDFTIKLIDF GSAAYLERGK LFYTFCGTIE YCAPEVLMGN PYRGPELEMW SLGVTLYTLV FEENPFCELE ETVEAAIHPP YLVSKELMSL VSGLLQPVPE RRTTLEKLVT DPWVTQPVNL ADYTWEEVFR VNKPESGVLS AASLEMGNRS LSDVAQAQEL CGGPVPGEAP NGQGCLHPGD PRLLTS (999-1323 aa encoded by BC063585 )

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.26 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 14396-1-AP (PASK antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293T cells were subjected to SDS PAGE followed by western blot with 14396-1-AP (PASK antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

K-562 cells were subjected to SDS PAGE followed by western blot with 14396-1-AP (PASK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

K-562 cells were subjected to SDS PAGE followed by western blot with 14396-1-AP (PASK antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HeLa cells using 14396-1-AP (PASK antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human prostate hyperplasia tissue slide using 14396-1-AP (PASK Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human prostate hyperplasia tissue slide using 14396-1-AP (PASK Antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human testis tissue slide using 14396-1-AP ( PASK Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human testis tissue slide using 14396-1-AP ( PASK Antibody) at dilution of 1:50 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-PASK (IP:14396-1-AP, 4ug; Detection:14396-1-AP 1:500) with K-562 cells lysate 3200ug.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-PASK (IP:14396-1-AP, 4 ug; Detection:14396-1-AP 1:300) with K-562 cells lysate 4000 ug.