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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB1741 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB1741, RRID:AB_1238109
- Product name
- PSMD4 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of PSMD4.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Structural studies of the interaction between ubiquitin family proteins and proteasome subunit S5a.
Hybrid proteasomes. Induction by interferon-gamma and contribution to ATP-dependent proteolysis.
Developmentally regulated, alternative splicing of the Rpn10 gene generates multiple forms of 26S proteasomes.
Walters KJ, Kleijnen MF, Goh AM, Wagner G, Howley PM
Biochemistry 2002 Feb 12;41(6):1767-77
Biochemistry 2002 Feb 12;41(6):1767-77
Hybrid proteasomes. Induction by interferon-gamma and contribution to ATP-dependent proteolysis.
Tanahashi N, Murakami Y, Minami Y, Shimbara N, Hendil KB, Tanaka K
The Journal of biological chemistry 2000 May 12;275(19):14336-45
The Journal of biological chemistry 2000 May 12;275(19):14336-45
Developmentally regulated, alternative splicing of the Rpn10 gene generates multiple forms of 26S proteasomes.
Kawahara H, Kasahara M, Nishiyama A, Ohsumi K, Goto T, Kishimoto T, Saeki Y, Yokosawa H, Shimbara N, Murata S, Chiba T, Suzuki K, Tanaka K
The EMBO journal 2000 Aug 1;19(15):4144-53
The EMBO journal 2000 Aug 1;19(15):4144-53
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The PSMD4 polyclonal antibody (Cat # PAB1741) is used in Western blot to detect PSMD4 in Jurkat cell lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with PSMD4 polyclonal antibody (Cat # PAB1741) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
