PA5-118210

antibody from Invitrogen Antibodies

Targeting: PSMD4 AF, AF-1, Rpn10, S5A

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-118210

Provider

Invitrogen Antibodies

Product name

PSMD4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Super Bright 780 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 780 nm. We recommend using a 780/60 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using PSMD4 Polyclonal Antibody (Product # PA5-118210) at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate, Lane B: hela Whole Cell Lysate, Lane C: 293T Whole Cell Lysate, Lane D: K562 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 40 kDa. Observed band size: 55 kDa. (We are unsure of the identity of these extra bands).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence staining of PSMD4 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with PSMD4 Polyclonal Antibody (Product # PA5-118210, 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to nucleus and cytoplasm.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human PSMD4 in human brain with PSMD4 Polyclonal Antibody (Product # PA5-118210, 1:100 dilution, formalin-fixed paraffin embedded sections).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human PSMD4 in human kidney with PSMD4 Polyclonal Antibody (Product # PA5-118210, 1:100 dilution, formalin-fixed paraffin embedded sections).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PSMD4 Immunoprecipitation using: Lane A: 0.5 mg 293T Whole Cell Lysate 4 µL with PSMD4 Polyclonal Antibody (Product # PA5-118210) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: PSMD4 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1,000dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 40 kDa. Observed band size: 55 kDa.