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Western blot
Immunocytochemistry
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Immunocytochemistry [1]
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Validation data
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- Product number
- BML-PW9250-0025 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#BML-PW9250-0025, RRID:AB_2052334
- Product name
- Proteasome 19S Rpn10/S5a subunit monoclonal antibody (S5a-18)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full length protein
- Reactivity
- Human, Rat, Bovine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- S5a-18
- Vial size
- 25 μl
- Storage
- -20°C
- Handling
- Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20¡C.
Submitted references Bortezomib Amplifies Effect on Intracellular Proteasomes by Changing Proteasome Structure.
The degradation of p53 and its major E3 ligase Mdm2 is differentially dependent on the proteasomal ubiquitin receptor S5a.
Pitcher DS, de Mattos-Shipley K, Tzortzis K, Auner HW, Karadimitris A, Kleijnen MF
EBioMedicine 2015 Jul;2(7):642-8
EBioMedicine 2015 Jul;2(7):642-8
The degradation of p53 and its major E3 ligase Mdm2 is differentially dependent on the proteasomal ubiquitin receptor S5a.
Sparks A, Dayal S, Das J, Robertson P, Menendez S, Saville MK
Oncogene 2014 Sep 18;33(38):4685-96
Oncogene 2014 Sep 18;33(38):4685-96
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Supportive validation
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Figure 1. Western blot analysis of Proteasome 19S Rpn10/S5a subunit, mAb (S5a-18) (Prod. No. BML-PW9250): Lane 1: MW marker, Lane 2: 3T3 (Prod. No. ADI-LYC-3T100), Lane 3: L929, Lane 4: PC-12 (Prod. No. ADI-LYC-PC100), Lane 5: MDBK, Lane 6: HeLa S100 fraction (Prod. No. BML-SW8750).
