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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [9]
- Other assay [1]
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- Product number
- UM800091 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#UM800091, RRID:AB_2629200
- Product name
- PD-1 (PDCD1) mouse monoclonal antibody,clone UMAB199
- Antibody type
- Monoclonal
- Description
- PD-1 (PDCD1) mouse monoclonal antibody,clone UMAB199
- Reactivity
- Canine
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- PDCD1
- Isotype
- IgG
- Antibody clone number
- UMAB199
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
Submitted references Immune Activation and Benefit From Avelumab in EBV-Positive Gastric Cancer.
Panda A, Mehnert JM, Hirshfield KM, Riedlinger G, Damare S, Saunders T, Kane M, Sokol L, Stein MN, Poplin E, Rodriguez-Rodriguez L, Silk AW, Aisner J, Chan N, Malhotra J, Frankel M, Kaufman HL, Ali S, Ross JS, White EP, Bhanot G, Ganesan S
Journal of the National Cancer Institute 2018 Mar 1;110(3):316-320
Journal of the National Cancer Institute 2018 Mar 1;110(3):316-320
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Thyroid, 7: Colon, 8: Spleen 9: Liver, 10: Ovary). Diluation: 1:500.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot of cell lysates (35ug) from 9 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549, 5: COS7, 6: Jurkat, 7: MDCK, 8: PC-12, 9: MCF7). Diluation: 1:500.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of PDCD1(RC210364)-stable-expression cells (left) labeling PDCD1 with mouse monoclonal antibody UM800091 (1:100, green) and nucleus with Hoechst33342 (blue) . HEK293T cells serve as negative control (right).
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunocytochemistry staining of PDCD1(RC210364)-stable-expression cells (left) labeling PDCD1 with mouse anti-PDCD1 monoclonal antibody UM800091 (1:900). The rihgt is negative control.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human tonsil using anti-PDCD1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 110C for 10 min, UM800091)(1:1200)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of 3 paraffin-embedded human lung carcinomas using anti-PD1 clone UMAB199 mouse monoclonal antibody at 1:800 requires HIER Accel [OriGene/GBI Labs B22-125] in Pressure Chamber for 3 minute on high. Detection of primary antibody was achieved with Polink2 Broad HRP DAB [OriGene/GBI Labs D22].
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human melanoma using anti-PD-1 clone UMAB199 mouse monoclonal antibody at 1:800 dilution of 1mg/mL and detection with Polink2 Broad HRP DAB. UM800091 requires heat-induced epitope retrieval with Accel for 3minutes at110C in pressure chamber . The composite image of 3 melanoma shows the tumor cells are negative for PD-1 however the activated TCells show strong membranous and cytoplasmic staining.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human ovarian carcinoma using anti-PD-1 clone UMAB199 mouse monoclonal antibody at 1:800 dilution of 1mg/mL and detection with Polink2 Broad HRP DAB. UM800091 requires heat-induced epitope retrieval with Accel for 3minutes at110C in pressure chamber . The image shows the tumor cells are negative for PD-1 however the activated TCells show strong membranous and cytoplasmic staining.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human prostate carcinoma using anti-PD-1 clone UMAB199 mouse monoclonal antibody at 1:800 dilution of 1mg/mL and detection with Polink2 Broad HRP DAB. UM800091 requires heat-induced epitope retrieval with Accel for 3minutes at110C in pressure chamber . The image shows the tumor cells are negative for PD-1 however the activated TCells show strong membranous and cytoplasmic staining.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human colon cancer using anti-PD-1 clone UMAB199 mouse monoclonal antibody at 1:800 dilution of 1mg/mL and detection with Polink2 Broad HRP DAB. UM800091 requires heat-induced epitope retrieval with Accel for 3minutes at110C in pressure chamber . The image shows the tumor cells are negative for PD-1 however the activated TCells show strong membranous and cytoplasmic staining.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human endometrial cancer using anti-PD-1 clone UMAB199 mouse monoclonal antibody at 1:800 dilution of 1mg/mL and detection with Polink2 Broad HRP DAB. UM800091 requires heat-induced epitope retrieval with Accel for 3minutes at110C in pressure chamber . The image shows the tumor cells are negative for PD-1 however the activated TCells show strong membranous and cytoplasmic staining.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Sequential double staining of paraffin human lung using anti-b-Catenin UM500015 (red) and anti-PD1 UM800091 (brown). Both antibodies at 1:800 dilution of 1mg/mL. Anti-PD1: heat-induced epitope retrieval with Accel; anti-b-Catenin: citrate pH6.0. Image shows tumor cells are strongly positve for b-catenin (red) and negative for PD1. The arrows point to the activated T cells (brown) showing strong membranous and cytoplasmic staining of PD1 and no staining with b-catenin.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Sequential double staining of paraffin human melanoma using b-catenin UM500015 (red) and PD1 UM800091 (brown). Both abs at 1:800 dilution of 1mg/mL; detection with Polink2 HRP DAB followed by Polink2 Broad AP. Anti-PD1: heat-induced epitope retrieval with Accel; anti-b-catenin: citrate pH6.0 . Image shows tumor cells are strongly positve for b-catenin (red) and negative for PD1. The activated T cells (brown) show strong membranous and cytoplasmic staining for PD1 and no staining with b-catenin.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-PDCD1 mouse monoclonal antibody (UM800091). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.(1:100)
- Validation comment
- 10K-CHIP
