CF806835

antibody from Invitrogen Antibodies

Targeting: PDCD1 CD279, hSLE1, PD-1, PD1, SLEB2

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

CF806835

Provider

Invitrogen Antibodies

Product name

PDCD1 Monoclonal Antibody (OTI8B1), TrueMAB™

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

OTI8B1

Vial size

100 µg

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDCD1 (RC210364, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-PDCD1. Positive lysates LY401555 (100 µg) and LC401555 (20 µg) can be purchased separately from OriGene.

ELISA

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PD1 ELISA with 1B11 Capture (CF807867) and 8B1 Detection (CF806835) Antibodies. Substrate used: Recombinant Human PD1 (TP310364)

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of PDCD1(RC210364)-stable-transfected HEK293T cells (left) labeling PDCD1 with mouse monoclonal antibody TA806835 (green) and nucleus with Hoechst33342 (blue). HEK293T cells serve as negative control (right). (1:100)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry staining of stable expression PD1 cells using anti-PDCD1 mouse monoclonal antibody (TA806835)(Left). The right is negative control. (1:100)(1:900)

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of stable expression PD1 cells using anti-PDCD1 antibody (TA806835) (Red) compared to a nonspecific negative control antibody (Blue). (1:50)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK293T cells transfected with either mouse PD1(MR227347) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PDCD1 antibody (TA806835),compared to a nonspecific negative control antibody (green),and then analyzed by flow cytometry. (1:50)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of stable expression PDL1 (RC213071) cells using anti-PDCD1 antibody (TA806835) (blue) or 0.3 µg/mL PD1-Fc fusion protein (TP700199) (green) or both (red),and detected by anti-Fc(human) IgG-FITC. (1:50)

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PD1 Luminex ELISA with 7B4 Capture (CF807995) and 8B1 Detection (CF806835) Antibodies. Substrate used: Recombinant Human PD1 (TP310364)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PD1 Luminex ELISA with 1B11 Capture (CF807867) and 8B1 Detection (CF806835) Antibodies. Substrate used: Recombinant Human PD1 (TP310364)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PD1 Luminex ELISA with 7B4 Capture (CF807995) and 8B1 Detection (CF806835) Antibodies. Substrate used: Recombinant Human PD1 (TP310364)