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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Flow cytometry [2]
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- Product number
- IC6006P-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse ROR gamma t/RORC2/NR1F3 PE-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human and mouse ROR gamma t/RORC2 in direct ELISAs. In direct ELISAs, no cross-reactivity with human and mouse RORC.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Antigen sequence
P51449- Isotype
- IgG
- Antibody clone number
- 600380
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references Itch inhibits IL-17-mediated colon inflammation and tumorigenesis by ROR-γt ubiquitination.
Pathogen-induced human TH17 cells produce IFN-γ or IL-10 and are regulated by IL-1β.
Kathania M, Khare P, Zeng M, Cantarel B, Zhang H, Ueno H, Venuprasad K
Nature immunology 2016 Aug;17(8):997-1004
Nature immunology 2016 Aug;17(8):997-1004
Pathogen-induced human TH17 cells produce IFN-γ or IL-10 and are regulated by IL-1β.
Zielinski CE, Mele F, Aschenbrenner D, Jarrossay D, Ronchi F, Gattorno M, Monticelli S, Lanzavecchia A, Sallusto F
Nature 2012 Apr 26;484(7395):514-8
Nature 2012 Apr 26;484(7395):514-8
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of ROR gamma t/RORC2/NR1F3 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with PMA, Calcium Ionomycin, LPS, and Recombinant Human IL-23 (Catalog # 1290-IL) were stained with Mouse Anti-Human/Mouse ROR gamma t/RORC2/ NR1F3 PE-conjugated Monoclonal Antibody (Catalog # IC6006P) and Mouse Anti-Human CD3 epsilon APC-conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on control antibody staining (Catalog # IC0041P). To facilitate intra-cellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of ROR gamma t/RORC2/NR1F3 in CD4+/CD8+ Mouse Thymocytes by Flow Cytometry. CD4+/CD8+ mouse thymocytes were stained with Mouse Anti-Human/Mouse ROR gamma t/RORC2/NR1F3 PE-conjugated Monoclonal Antibody (Catalog # IC6006P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
