MAB4874

antibody from R&D Systems

Targeting: S100A11 S100C

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB4874

Provider

R&D Systems

Product name

Human S100A11 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human S100A11 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) S100A1, A2, A4, A6, A7, A8, A9, A10, A13, rhS100B, rhS100P, recombinant mouse S100A1, A4, A6, A8, A9, A10, A11, A13, or A16 is observed.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

P31949


Isotype

IgG

Antibody clone number

672816

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human S100A11 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF Membrane was probed with 2 µg/mL of Human S100A11 Monoclonal Antibody (Catalog # MAB4874) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for S100A11 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.