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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunoprecipitation [1]
- Chromatin Immunoprecipitation [1]
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Validation data
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- Product number
- GTX107234 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX107234, RRID:AB_1950657
- Product name
- L3MBTL2 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30?g whole cell lysate)A:MOLT4 (GTX27912)7.5% SDS PAGEGTX107234 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Whole cell extract (30 ?g) was separated by 7.5% SDS-PAGE, and the membrane was blotted with L3MBTL2 antibody (GTX107234) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Mouse tissue extract (50 ?g) was separated by 7.5% SDS-PAGE, and the membrane was blotted with L3MBTL2 antibody (GTX107234) diluted at 1:500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- L3MBTL2 antibody immunoprecipitates L3MBTL2 protein in IP experiments. IP Sample: 1000 ?g HeLa whole cell lysate/extract A. 40 £gg HeLa whole cell lysate/extract B. Control with 2.5 £gg of preimmune rabbit IgG C. Immunoprecipitation of L3MBTL2 protein by 2.5 £gg of L3MBTL2 antibody (GTX107234) 5% SDS-PAGE The immunoprecipitated L3MBTL2 protein was detected by L3MBTL2 antibody (GTX107234) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- L3MBTL2 antibody immunoprecipitates L3MBTL2 protein-DNA in ChIP experiments. ChIP Sample: HeLa whole cell lysate/extract A. 5 £gg preimmune rabbit IgG B. 5 £gg of L3MBTL2 antibody (GTX107234) The precipitated DNA was detected by PCR with primer set targeting to CDC7 gene locus.
