NBP2-20019

antibody from Novus Biologicals

Targeting: PYGO2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

NBP2-20019

Provider

Novus Biologicals

Product name

Rabbit Polyclonal Pygopus-2 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Pygopus 2 Antibody [NBP2-20019] - Sample (30 ug of whole cell lysate) A:NIH-3T310% SDS PAGE gel, diluted at 1:1000.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Pygopus-2 Antibody [NBP2-20019] - Pygopus-2 was immunoprecipitated from 5*107 cells of SW480 whole cell lysate using 1 ug of Pygopus-2 antibody. The precipitated Pygopus-2 was detected by Pygopus-2 Antibody diluted at 1:15000. A: IgG. B: Pygopus-2 antibody

Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: Pygopus 2 Antibody [NBP2-20019] - Pygopus 2 was immunoprecipitated from 5*10^7 cells of SW480 whole cell lysate using 1 ug of anti-pygopus 2 antibody. The precipitated pygopus 2 was detected by NBP2-20019 diluted at 1:15000. A: IP-IgG B: IP-pygopus 2 antibody.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Pygopus 2 Antibody [NBP2-20019] - Sample: Paraffin-embedded Lung adenocarcinoma. antibody dilution: 1:500.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Pygopus-2 Antibody [NBP2-20019] - Pygopus-2 antibody detects Pygopus-2 protein at nucleus in mouse stomach by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. Pygopus-2 antibody diluted at 1:500.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Pygopus-2 Antibody [NBP2-20019] - Pygopus-2 antibody detects Pygopus-2 protein at nucleus in rat intestine by immunohistochemical analysis. Sample: Paraffin-embedded rat intestine. Pygopus-2 antibody diluted at 1:500.