Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- MA3-902 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HCN3 Monoclonal Antibody (TLL6C5)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- MA3-902 detects HCN3 from human, mouse and rat samples.
- Antibody clone number
- TLL6C5
- Concentration
- Conc. Not Determined
Submitted references Thalamocortical neurons display suppressed burst-firing due to an enhanced Ih current in a genetic model of absence epilepsy.
HCN channels are expressed differentially in retinal bipolar cells and concentrated at synaptic terminals.
Hyperpolarization-activated channels HCN1 and HCN4 mediate responses to sour stimuli.
Cain SM, Tyson JR, Jones KL, Snutch TP
Pflugers Archiv : European journal of physiology 2015 Jun;467(6):1367-82
Pflugers Archiv : European journal of physiology 2015 Jun;467(6):1367-82
HCN channels are expressed differentially in retinal bipolar cells and concentrated at synaptic terminals.
Müller F, Scholten A, Ivanova E, Haverkamp S, Kremmer E, Kaupp UB
The European journal of neuroscience 2003 May;17(10):2084-96
The European journal of neuroscience 2003 May;17(10):2084-96
Hyperpolarization-activated channels HCN1 and HCN4 mediate responses to sour stimuli.
Stevens DR, Seifert R, Bufe B, Müller F, Kremmer E, Gauss R, Meyerhof W, Kaupp UB, Lindemann B
Nature 2001 Oct 11;413(6856):631-5
Nature 2001 Oct 11;413(6856):631-5
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on normal deparaffinized Human brain tissue tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a rat monoclonal antibody recognizing Anti-HCN3 (Product # MA3-902) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on cancer biopsies of deparaffinized Human colon carcinoma tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a rat monoclonal antibody recognizing Anti-HCN3 (Product # MA3-902) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 4 HCN protein isoforms are differentially expressed in GAERS VB thalamus. a Representative Western blots for HCN proteins from adult NEC and GAERS VB samples. Primary antibodies to detect each of the HCN channel isoforms were compared to vinculin in order to normalize the expression data. b Mean Western blot densitometry data for HCN1-4 normalized to vinculin for NEC ( n = 12 samples (six animals); black columns ) and GAERS ( n = 12 samples (six animals); grey columns ) VB samples