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Western blot
Immunocytochemistry
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
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Validation data
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- Product number
- 200-301-F42 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#200-301-F42, RRID:AB_2611162
- Product name
- Anti-HCN3 (MOUSE) Monoclonal Antibody - 200-301-F42
- Antibody type
- Monoclonal
- Description
- Anti-HCN3 Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with HCN3 from Human, Rat, and Mouse based on 100% homology with the immunizing sequence. No cross-reactivity against other HCNs. Cross-reactivity with HCN3 from other sources has not been determined. Channels and Transporters research.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- S141-28
- Vial size
- 100 µl
- Concentration
- 1.0 mg/ml
- Storage
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Handling
- Dry Ice
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Supportive validation
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- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Immunofluorescence of mouse anti-HCN3 antibody. Tissue: human hippocampal tissues. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary Antibody: HCN3 antibody at 10ug/ml for 1h at RT. Secondary antibody: Fluorescein mouse secondary at 1:10,000 for 45 min at RT. Localization: Nuclear. Staining: green fluorescent signal.
- Sample type
- IF Microscopy
