Explore
Validate
LearnMA5-34750
antibody from Invitrogen Antibodies
Targeting: ARHGEF2
GEF-H1, GEFH1, KIAA0651, Lfc, LFP40, P40
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [2]
- Immunohistochemistry [3]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA5-34750 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GEF-H1 Recombinant Rabbit Monoclonal Antibody (JG36-46)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Positive Control: 293T, A431, HUVEC, SH-SY-5Y, rat brain tissue, human tonsil tissue, mouse testis tissue.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- JG36-46
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles, store in dark
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of GEF H1 in SH-SY-5Y cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with GEF H1 monoclonal antibody (Product # MA5-34750), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of GEF H1 in HUVEC cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with GEF H1 monoclonal antibody (Product # MA5-34750), followed by DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of GEF H1 in paraffin-embedded rat brain tissue. Samples were incubated with GEF H1 monoclonal antibody (Product # MA5-34750), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of GEF H1 in paraffin-embedded mouse testis tissue. Samples were incubated with GEF H1 monoclonal antibody (Product # MA5-34750), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of GEF H1 in paraffin-embedded human tonsil tissue. Samples were incubated with GEF H1 monoclonal antibody (Product # MA5-34750), and followed by hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of GEF H1 in HL-60 cells (yellow) compared with an unlabelled control (cells without incubation with primary antibody; purple). Samples were incubated with GEF H1 monoclonal antibody (Product # MA5-34750) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.
