10278-1-AP

antibody from Invitrogen Antibodies

Targeting: SSR2 TLAP, TRAPB

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

10278-1-AP

Provider

Invitrogen Antibodies

Product name

TRAPB/SSR2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MRLLSFVVL ALFAVTQAEE GARLLASKSL LNRYAVEGRD LTLQYNIYNV GSSAALDVEL SDDSFPPEDF GIVSGMLNVK WDRIAPASNV SHTVVLRPLK AGYFNFTSAT ITYLAQEDGP VVIGSTSAPG QGGILAQREF DRRFSPHFLD WAAFGVMTLP SIGIPLLLWY SSKRKY (1-175 aa encoded by B C000341)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.29 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 10278-1-AP (TRAPB/SSR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

L02 cells were subjected to SDS PAGE followed by western blot with 10278-1-AP (TRAPB/SSR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse liver tissue were subjected to SDS PAGE followed by western blot with 10278-1-AP (TRAPB/SSR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells using 10278-1-AP (TRAPB/SSR2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer using 10278-1-AP (TRAPB/SSR2 antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer using 10278-1-AP (TRAPB/SSR2 antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human pancreas slide using 10278-1-AP (TRAPB/SSR2 antibody at dilution of 1:50.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human pancreas slide using 10278-1-AP (TRAPB/SSR2 antibody at dilution of 1:50.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 MCF-7 cells were stained with 0.2ug TRAPB/SSR2 antibody (10278-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.