LS-C820597

antibody from LSBio

Targeting: SEMA4A CORD10, FLJ12287, SEMAB, SemB

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunoprecipitation (Enhanced validation)

Antibody Data

Product number

LS-C820597

Provider

LSBio

Product name

SEMA4A / Semaphorin 4A Antibody (Preservative Free) LS-C820597

Antibody type

Polyclonal

Description

Protein A affinity chromatography

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.

Immunoprecipitation

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

SEMA4A was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. 0.5 uL anti-SEMA4A rabbit polyclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-SEMA4A rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 84 kDa. Observed band size: 105 kDa.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

SEMA4A was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. 0.5 uL anti-SEMA4A rabbit polyclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-SEMA4A rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 84 kDa. Observed band size: 105 kDa.