LS-C814965

antibody from LSBio

Targeting: ALDH18A1 GSAS, P5CS, PYCS, SPG9

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C814965

Provider

LSBio

Product name

ALDH18A1 Antibody (Preservative Free) LS-C814965

Antibody type

Polyclonal

Description

Protein A affinity chromatography

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Anti-ALDH18A1 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: 293T Whole Cell Lysate. Lane C: Jurkat Whole Cell Lysate. Lane D: MCF7 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 87 kDa. Observed band size: 87 kDa.

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

ALDH18A1 was immunoprecipitated using: Lane A: 0.5 mg MCF7 Whole Cell Lysate. Lane B: 0.5 mg 293T Whole Cell Lysate. 4 uL anti-ALDH18A1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-ALDH18A1 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 87 kDa. Observed band size: 90 kDa.