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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- HPA008333 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA008333, RRID:AB_1078125
- Product name
- Anti-ALDH18A1
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
SIVEQVSVLQNQGREMMLVTSGAVAFGKQRLRHEI
LLSQSVRQALHSGQNQLKEMAIPVLEARACAAAGQ
SGLMALYEAMFTQYSICAAQILVTNLDFHDEQKRR
NLNGTLHELLRMNIVPIVNTNDAVVPPAEPNSDLQ
GVNVISVKD- Isotype
- IgG
- Vial size
- 100 µl
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Submitted references Nuclear cytoplasmic trafficking of proteins is a major response of human fibroblasts to oxidative stress.
Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy
Baqader NO, Radulovic M, Crawford M, Stoeber K, Godovac-Zimmermann J
Journal of proteome research 2014 Oct 3;13(10):4398-423
Journal of proteome research 2014 Oct 3;13(10):4398-423
Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy
Stadler C, Hjelmare M, Neumann B, Jonasson K, Pepperkok R, Uhlén M, Lundberg E
Journal of Proteomics 2012 April;75(7):2236-2251
Journal of Proteomics 2012 April;75(7):2236-2251
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Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ALDH18A1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.
- Sample type
- HUMAN
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.
- Sample type
- HUMAN
