Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
Reference
Comment
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- Product number
- TA504372 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA504372, RRID:AB_11126685
- Product name
- SH2D2A mouse monoclonal antibody, clone OTI3C7 (formerly 3C7)
- Antibody type
- Monoclonal
- Description
- SH2D2A mouse monoclonal antibody, clone OTI3C7 (formerly 3C7)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- SH2D2A
- Isotype
- IgG
- Antibody clone number
- OTI3C7
- Vial size
- 100 µl
- Concentration
- 0.75 mg/ml
Submitted references Expression of the T cell-specific adapter protein in human tissues.
Pandya AD, Leergaard TB, Dissen E, Haraldsen G, Spurkland A
Scandinavian journal of immunology 2014 Sep;80(3):169-79
Scandinavian journal of immunology 2014 Sep;80(3):169-79
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SH2D2A monoclonal antibody.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SH2D2A (RC204162, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SH2D2A.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-SH2D2A monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-SH2D2A mouse monoclonal antibody (TA504372) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SH2D2A(RC204162).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Figure from citation: Comparison of IHC on tonsil with clone 4F3(TA504364) and clone 3C7(TA504372) SH2D2A mAb and HRP-conjugated secondary antibodies.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC204162 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SH2D2A antibody(TA504372), and then analyzed by flow cytometry.
- Validation comment
- FC