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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB3531 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB3531, RRID:AB_1575116
- Product name
- GCK polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of GCK.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Insights into the biochemical and genetic basis of glucokinase activation from naturally occurring hypoglycemia mutations.
Genetic epidemiology of MODY in the Czech republic: new mutations in the MODY genes HNF-4alpha, GCK and HNF-1alpha.
A functional link between glucokinase binding to insulin granules and conformational alterations in response to glucose and insulin.
Gloyn AL, Noordam K, Willemsen MA, Ellard S, Lam WW, Campbell IW, Midgley P, Shiota C, Buettger C, Magnuson MA, Matschinsky FM, Hattersley AT
Diabetes 2003 Sep;52(9):2433-40
Diabetes 2003 Sep;52(9):2433-40
Genetic epidemiology of MODY in the Czech republic: new mutations in the MODY genes HNF-4alpha, GCK and HNF-1alpha.
Pruhova S, Ek J, Lebl J, Sumnik Z, Saudek F, Andel M, Pedersen O, Hansen T
Diabetologia 2003 Feb;46(2):291-5
Diabetologia 2003 Feb;46(2):291-5
A functional link between glucokinase binding to insulin granules and conformational alterations in response to glucose and insulin.
Rizzo MA, Magnuson MA, Drain PF, Piston DW
The Journal of biological chemistry 2002 Sep 13;277(37):34168-75
The Journal of biological chemistry 2002 Sep 13;277(37):34168-75
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The GCK polyclonal antibody (Cat # PAB3531) is used in Western blot to detect GCK in HepG2 cell lysate (Lane 1) and mouse liver tissue lysate (Lane 2) .
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with GCK polyclonal antibody (Cat # PAB3531) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
