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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunohistochemistry [1]
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- Product number
- NBP1-33144 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-33144, RRID:AB_2107633
- Product name
- Rabbit Polyclonal Glucokinase/GCK Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Glucokinase/GCK Antibody [NBP1-33144] - Sample (30 ug of whole cell lysate) A: H1299 B: Hela 10% SDS PAGE; antibody diluted at 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Glucokinase/GCK Antibody [NBP1-33144] - A. 30 ug Huh7 whole cell lysate/extract B. 30 ug Hep3B whole cell lysate/extract C. 30 ug HepG2 whole cell lysate/extract 10% SDS-PAGE GCK antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Glucokinase/GCK Antibody [NBP1-33144] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Glucokinase antibody [N1C1-2] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Glucokinase/GCK Antibody [NBP1-33144] - MCF7 xenograft, using Glucokinase antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Glucokinase/GCK Antibody [NBP1-33144] - MCF7 xenograft, using Glucokinase antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
