Explore
Validate
Learn
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunoprecipitation [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- H00000922-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00000922-M01, RRID:AB_537566
- Product name
- Mouse Monoclonal CD5L Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. CD5L - CD5 antigen-like (scavenger receptor cysteine rich family)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references CD5L is upregulated in hepatocellular carcinoma and promotes liver cancer cell proliferation and antiapoptotic responses by binding to HSPA5 (GRP78).
Proteomic analysis of microvesicles from plasma of healthy donors reveals high individual variability.
Aran G, Sanjurjo L, Bárcena C, Simon-Coma M, Téllez É, Vázquez-Vitali M, Garrido M, Guerra L, Díaz E, Ojanguren I, Elortza F, Planas R, Sala M, Armengol C, Sarrias MR
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2018 Jul;32(7):3878-3891
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2018 Jul;32(7):3878-3891
Proteomic analysis of microvesicles from plasma of healthy donors reveals high individual variability.
Bastos-Amador P, Royo F, Gonzalez E, Conde-Vancells J, Palomo-Diez L, Borras FE, Falcon-Perez JM
Journal of proteomics 2012 Jun 27;75(12):3574-84
Journal of proteomics 2012 Jun 27;75(12):3574-84
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CD5L Antibody (1C8) [H00000922-M01] - Analysis of CD5L expression in transfected 293T cell line by CD5L monoclonal antibody (M01), clone 1C8. Lane 1: CD5L transfected lysate (38 KDa). Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CD5L Antibody (1C8) [H00000922-M01] - CD5L monoclonal antibody (M01), clone 1C8. Analysis of CD5L expression in different cell lines.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: CD5L Antibody (1C8) [H00000922-M01] - Detection limit for recombinant GST tagged CD5L is approximately 0.3ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: CD5L Antibody (1C8) [H00000922-M01] - Analysis of CD5L transfected lysate using anti-CD5L monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CD5L MaxPab rabbit polyclonal antibody.
