Antibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Flow cytometry [2]
- Other assay [1]
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- Product number
- 17-0015-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD1c Monoclonal Antibody (L161), APC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This L161 monoclonal antibody detects CD1c (also known as BDCA-1), a glycoprotein that is noncovalently linked to beta-2 microglobulin on thymocytes and antigen presenting cells such as dendritic and Langerhans cells. This molecule is also expressed on some circulating and marginal zone B cells, as well as in lymph nodes and germinal centers. CD1c is involved in the presentation of lipid antigens such as microbial fatty acids to effector T cells during the adaptive immune response. Finally, alternative splicing gives rise to three different isoforms of CD1c (soluble, membrane, and cytoplasmic/soluble isoforms). Applications Reported: This L161 antibody has been reported for use in flow cytometric analysis. Applications Tested: This L161 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- L161
- Vial size
- 100 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Dendritic cells' characteristics in patients with treated systemic lupus erythematosus.
STAT3 Inhibition Combined with CpG Immunostimulation Activates Antitumor Immunity to Eradicate Genetically Distinct Castration-Resistant Prostate Cancers.
Malaria-induced NLRP12/NLRP3-dependent caspase-1 activation mediates inflammation and hypersensitivity to bacterial superinfection.
CD36 contributes to malaria parasite-induced pro-inflammatory cytokine production and NK and T cell activation by dendritic cells.
CD1 antigen presentation: how it works.
Membrane trafficking of CD1c on activated T cells.
Human CD1b and CD1c isoforms survey different intracellular compartments for the presentation of microbial lipid antigens.
BDCA-2, BDCA-3, and BDCA-4: three markers for distinct subsets of dendritic cells in human peripheral blood.
Wardowska A, Smoleńska Ż, Lisowska KA, Zdrojewski Z, Pikuła M
Acta biochimica Polonica 2020 Jul 30;67(3):417-429
Acta biochimica Polonica 2020 Jul 30;67(3):417-429
STAT3 Inhibition Combined with CpG Immunostimulation Activates Antitumor Immunity to Eradicate Genetically Distinct Castration-Resistant Prostate Cancers.
Moreira D, Adamus T, Zhao X, Su YL, Zhang Z, White SV, Swiderski P, Lu X, DePinho RA, Pal SK, Kortylewski M
Clinical cancer research : an official journal of the American Association for Cancer Research 2018 Dec 1;24(23):5948-5962
Clinical cancer research : an official journal of the American Association for Cancer Research 2018 Dec 1;24(23):5948-5962
Malaria-induced NLRP12/NLRP3-dependent caspase-1 activation mediates inflammation and hypersensitivity to bacterial superinfection.
Ataide MA, Andrade WA, Zamboni DS, Wang D, Souza Mdo C, Franklin BS, Elian S, Martins FS, Pereira D, Reed G, Fitzgerald KA, Golenbock DT, Gazzinelli RT
PLoS pathogens 2014 Jan;10(1):e1003885
PLoS pathogens 2014 Jan;10(1):e1003885
CD36 contributes to malaria parasite-induced pro-inflammatory cytokine production and NK and T cell activation by dendritic cells.
Gowda NM, Wu X, Kumar S, Febbraio M, Gowda DC
PloS one 2013;8(10):e77604
PloS one 2013;8(10):e77604
CD1 antigen presentation: how it works.
Barral DC, Brenner MB
Nature reviews. Immunology 2007 Dec;7(12):929-41
Nature reviews. Immunology 2007 Dec;7(12):929-41
Membrane trafficking of CD1c on activated T cells.
del C Salamone M, Mendiguren AK, Salamone GV, Fainboim L
Journal of leukocyte biology 2001 Oct;70(4):567-77
Journal of leukocyte biology 2001 Oct;70(4):567-77
Human CD1b and CD1c isoforms survey different intracellular compartments for the presentation of microbial lipid antigens.
Briken V, Jackman RM, Watts GF, Rogers RA, Porcelli SA
The Journal of experimental medicine 2000 Jul 17;192(2):281-8
The Journal of experimental medicine 2000 Jul 17;192(2):281-8
BDCA-2, BDCA-3, and BDCA-4: three markers for distinct subsets of dendritic cells in human peripheral blood.
Dzionek A, Fuchs A, Schmidt P, Cremer S, Zysk M, Miltenyi S, Buck DW, Schmitz J
Journal of immunology (Baltimore, Md. : 1950) 2000 Dec 1;165(11):6037-46
Journal of immunology (Baltimore, Md. : 1950) 2000 Dec 1;165(11):6037-46
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD19 eFluor® 450 (Product # 48-0199-42) and Mouse IgG1 K Isotype Control APC (Product # 17-4714-81) (left) or Anti-Human CD1c APC (right). Cells in the lymphocyte gate were used for analysis.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD19 eFluor® 450 (Product # 48-0199-42) and Mouse IgG1 K Isotype Control APC (Product # 17-4714-81) (left) or Anti-Human CD1c APC (right). Cells in the lymphocyte gate were used for analysis.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL