MA1-4997

antibody from Invitrogen Antibodies

Targeting: FCER1A FCE1A

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-4997

Provider

Invitrogen Antibodies

Product name

FceR1 alpha Monoclonal Antibody (CRA1 (AER37))

Antibody type

Monoclonal

Antigen

Other

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

CRA1 (AER37)

Vial size

100 µg

Concentration

1 mg/mL

Storage

-20°C

References

Submitted references

Carbonic anhydrase enzymes regulate mast cell-mediated inflammation.

Henry EK, Sy CB, Inclan-Rico JM, Espinosa V, Ghanny SS, Dwyer DF, Soteropoulos P, Rivera A, Siracusa MC
The Journal of experimental medicine 2016 Aug 22;213(9):1663-73

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NADPH oxidase-independent formation of extracellular DNA traps by basophils.

Morshed M, Hlushchuk R, Simon D, Walls AF, Obata-Ninomiya K, Karasuyama H, Djonov V, Eggel A, Kaufmann T, Simon HU, Yousefi S
Journal of immunology (Baltimore, Md. : 1950) 2014 Jun 1;192(11):5314-23

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of FceR1 alpha in TH-2 induced neutrophils using an FceR1 alpha monoclonal antibody (Product # MA1-4997). FceR1 alpha expression induced by TH-2 cytokines in neutrophils from allergic asthmatics. Total protein lysates were subjected to immunoprecipitation with IgE/anti IgE and Western blotting with (Product # MA1-4997). Basophilic cell line (KU812) was used as positive control. Negative control corresponds to neutrophil protein lysate analyzed without IgE/anti-IgE immunoprecipitation. (Image from Alphonse MP et al. PLoS One. 2008 Apr 2;3(4): e1921.)

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow-cytometry of CHO/Fc epsilon R1 alpha cells with FceR1 alpha Monoclonal Antibody (Product # MA1-4997, CRA1) and FceR1 alpha Monoclonal Antibody (Product # MA1-4998, CRA2). CHO cells were transfected with plasmid expressing human Fc epsilon RI alpha.The second antibody is FITC-conjugated anti-mouse IgG2b antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow-cytometry of CHO/Fc epsilon R1 alpha cells with FceR1 alpha Monoclonal Antibody (Product # MA1-4997, CRA1) and FceR1 alpha Monoclonal Antibody (Product # MA1-4998, CRA2). CHO cells were transfected with plasmid expressing human Fc epsilon RI alpha.The second antibody is FITC-conjugated anti-mouse IgG2b antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow-cytometry of CHO/Fc epsilon R1 alpha cells with FceR1 alpha Monoclonal Antibody (Product # MA1-4997, CRA1) and FceR1 alpha Monoclonal Antibody (Product # MA1-4998, CRA2). CHO cells were transfected with plasmid expressing human Fc epsilon RI alpha.The second antibody is FITC-conjugated anti-mouse IgG2b antibody.