Antibody data
- Antibody Data
- Antigen structure
- References [14]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 12-5899-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FceR1 alpha Monoclonal Antibody (AER-37 (CRA1)), PE, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The AER-37 monoclonal antibody reacts with the Fc epsilon RI alpha subunit, an IgE-binding subunit lacking signal-transducing ability. Fc epsilon RI apha is expressed on mast and basophil cells and is upregulated by the presence of IgE. Fc epsilon RI alpha forms a tetrameric complex with one beta and two gamma subunits. The beta and gamma subunits possess immunoreceptor tyrosine-based activation motifs (ITAM). The Fc epsilon RI complex plays an important role in triggering IgE-mediated allergic reactions. Applications Reported: This AER-37 (CRA1) antibody has been reported for use in flow cytometric analysis. Applications Tested: This AER-37 (CRA1) antibody has been pre-titrated and tested by flow cytometric analysis of peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Isotype
- IgG
- Antibody clone number
- AER-37 (CRA1)
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references The Humanised NPY-mRFP RBL Reporter Cell Line Is a Fast and Inexpensive Tool for Detection of Allergen-Specific IgE in Human Sera.
Human Mast Cell Proteome Reveals Unique Lineage, Putative Functions, and Structural Basis for Cell Ablation.
MRGPRX2 activation as a rapid, high-throughput mechanistic-based approach for detecting peptide-mediated human mast cell degranulation liabilities.
Type 2 innate lymphoid cells participate in IL-33-stimulated Th2-associated immune response in chronic obstructive pulmonary disease.
Aryl Hydrocarbon Receptor Controls Monocyte Differentiation into Dendritic Cells versus Macrophages.
Dendritic cell-bound IgE functions to restrain allergic inflammation at mucosal sites.
A single glycan on IgE is indispensable for initiation of anaphylaxis.
Development of β-lactoglobulin-specific chimeric human IgEκ monoclonal antibodies for in vitro safety assessment of whey hydrolysates.
MHC class II expression in human basophils: induction and lack of functional significance.
The signal peptide of the IgE receptor alpha-chain prevents surface expression of an immunoreceptor tyrosine-based activation motif-free receptor pool.
Selective impairment of Toll-like receptor 2-mediated proinflammatory cytokine production by monocytes from patients with atopic dermatitis.
IgE- and FcepsilonRI-mediated migration of human basophils.
Omalizumab treatment downregulates dendritic cell FcepsilonRI expression.
IgE enhances Fc epsilon receptor I expression and IgE-dependent release of histamine and lipid mediators from human umbilical cord blood-derived mast cells: synergistic effect of IL-4 and IgE on human mast cell Fc epsilon receptor I expression and mediator release.
Prakash PS, Barwary NJS, Weber MHW, Wan D, Conejeros I, Pereira Moreira B, Alharbi WS, van Hellemond JJ, Akinwale J, Falcone FH
Diagnostics (Basel, Switzerland) 2022 Aug 25;12(9)
Diagnostics (Basel, Switzerland) 2022 Aug 25;12(9)
Human Mast Cell Proteome Reveals Unique Lineage, Putative Functions, and Structural Basis for Cell Ablation.
Plum T, Wang X, Rettel M, Krijgsveld J, Feyerabend TB, Rodewald HR
Immunity 2020 Feb 18;52(2):404-416.e5
Immunity 2020 Feb 18;52(2):404-416.e5
MRGPRX2 activation as a rapid, high-throughput mechanistic-based approach for detecting peptide-mediated human mast cell degranulation liabilities.
Lafleur MA, Werner J, Fort M, Lobenhofer EK, Balazs M, Goyos A
Journal of immunotoxicology 2020 Dec;17(1):110-121
Journal of immunotoxicology 2020 Dec;17(1):110-121
Type 2 innate lymphoid cells participate in IL-33-stimulated Th2-associated immune response in chronic obstructive pulmonary disease.
Jiang M, Tao S, Zhang S, Wang J, Zhang F, Li F, Ding J
Experimental and therapeutic medicine 2019 Oct;18(4):3109-3116
Experimental and therapeutic medicine 2019 Oct;18(4):3109-3116
Aryl Hydrocarbon Receptor Controls Monocyte Differentiation into Dendritic Cells versus Macrophages.
Goudot C, Coillard A, Villani AC, Gueguen P, Cros A, Sarkizova S, Tang-Huau TL, Bohec M, Baulande S, Hacohen N, Amigorena S, Segura E
Immunity 2017 Sep 19;47(3):582-596.e6
Immunity 2017 Sep 19;47(3):582-596.e6
Dendritic cell-bound IgE functions to restrain allergic inflammation at mucosal sites.
Platzer B, Baker K, Vera MP, Singer K, Panduro M, Lexmond WS, Turner D, Vargas SO, Kinet JP, Maurer D, Baron RM, Blumberg RS, Fiebiger E
Mucosal immunology 2015 May;8(3):516-32
Mucosal immunology 2015 May;8(3):516-32
A single glycan on IgE is indispensable for initiation of anaphylaxis.
Shade KT, Platzer B, Washburn N, Mani V, Bartsch YC, Conroy M, Pagan JD, Bosques C, Mempel TR, Fiebiger E, Anthony RM
The Journal of experimental medicine 2015 Apr 6;212(4):457-67
The Journal of experimental medicine 2015 Apr 6;212(4):457-67
Development of β-lactoglobulin-specific chimeric human IgEκ monoclonal antibodies for in vitro safety assessment of whey hydrolysates.
Knipping K, Simons PJ, Buelens-Sleumer LS, Cox L, den Hartog M, de Jong N, Teshima R, Garssen J, Boon L, Knippels LM
PloS one 2014;9(8):e106025
PloS one 2014;9(8):e106025
MHC class II expression in human basophils: induction and lack of functional significance.
Voskamp AL, Prickett SR, Mackay F, Rolland JM, O'Hehir RE
PloS one 2013;8(12):e81777
PloS one 2013;8(12):e81777
The signal peptide of the IgE receptor alpha-chain prevents surface expression of an immunoreceptor tyrosine-based activation motif-free receptor pool.
Platzer B, Fiebiger E
The Journal of biological chemistry 2010 May 14;285(20):15314-15323
The Journal of biological chemistry 2010 May 14;285(20):15314-15323
Selective impairment of Toll-like receptor 2-mediated proinflammatory cytokine production by monocytes from patients with atopic dermatitis.
Hasannejad H, Takahashi R, Kimishima M, Hayakawa K, Shiohara T
The Journal of allergy and clinical immunology 2007 Jul;120(1):69-75
The Journal of allergy and clinical immunology 2007 Jul;120(1):69-75
IgE- and FcepsilonRI-mediated migration of human basophils.
Suzukawa M, Hirai K, Iikura M, Nagase H, Komiya A, Yoshimura-Uchiyama C, Yamada H, Ra C, Ohta K, Yamamoto K, Yamaguchi M
International immunology 2005 Sep;17(9):1249-55
International immunology 2005 Sep;17(9):1249-55
Omalizumab treatment downregulates dendritic cell FcepsilonRI expression.
Prussin C, Griffith DT, Boesel KM, Lin H, Foster B, Casale TB
The Journal of allergy and clinical immunology 2003 Dec;112(6):1147-54
The Journal of allergy and clinical immunology 2003 Dec;112(6):1147-54
IgE enhances Fc epsilon receptor I expression and IgE-dependent release of histamine and lipid mediators from human umbilical cord blood-derived mast cells: synergistic effect of IL-4 and IgE on human mast cell Fc epsilon receptor I expression and mediator release.
Yamaguchi M, Sayama K, Yano K, Lantz CS, Noben-Trauth N, Ra C, Costa JJ, Galli SJ
Journal of immunology (Baltimore, Md. : 1950) 1999 May 1;162(9):5455-65
Journal of immunology (Baltimore, Md. : 1950) 1999 May 1;162(9):5455-65
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human IgE FITC (Product # 11-6986-42) and Mouse IgG2b K Isotype Control PE (Product # 12-4732-81) (left) or Anti-Human Fc epsilon Receptor I alpha (FceR1) PE (right). Cells in the lymphocyte gate were used for analysis (note: basophils reside in FCS/SSC position of lymphocytes).
- Conjugate
- Yellow dye